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当前位置: 首页 > 文章 > 马铃薯纺锤块茎类病毒内蒙古分离株的克隆及序列分析 中国马铃薯 2016 (1) 31-38
Position: Home > Articles > Cloning and Sequence Analysis of Potato Spindle Tuber Viroid Isolate from Inner Mongolia Chinese Potato Journal 2016 (1) 31-38

马铃薯纺锤块茎类病毒内蒙古分离株的克隆及序列分析

作  者：
王建;迟胜起;韩磊;张剑峰;梁文星
单  位：
青岛农业大学农学与植保学院
关键词：
马铃薯纺锤块茎类病毒;反转录-聚合酶链式反应;序列分析
摘  要：
采用内蒙古自治区采集的感染马铃薯纺锤块茎类病毒(Potato spindle tuber viroid,PSTVd)的马铃薯材料,克隆了PSTVd的全长基因,命名为内蒙古分离株(PSTVd-IM),对其序列进行分析。结果表明,该序列大小为359 bp,其与俄罗斯的PSTVd分离株(EF044305.1)序列吻合。将该分离物与国内外已经报道的PSTVd株系进行了比较,构建了系统进化树,确定了其分类地位;通过序列比对和二级结构分析,发现PSTVd-IM与已报道的PSTVd三类致病类型株系:强株系(U23058.1)、中间株系(AY937179.1)和弱株系(M14814.1)的同源性分别为97.78%,98.61%和99.44%;且与这三种类型株系在中央保守区、致病区和可变区结构域存在差异,说明这些结构域可能与其致病性相关。PSTVd-IM的序列与二级结构分析有利于明确其来源及致病性的差异位点,对于PSTVd的致病机制的研究具有重要意义。
译  名：
Cloning and Sequence Analysis of Potato Spindle Tuber Viroid Isolate from Inner Mongolia
作  者：
WANG Jian;CHI Shengqi;HAN Lei;ZHANG Jianfeng;LIANG Wenxing;College of Agronomy and Plant Protection, Qingdao Agricultural University;
关键词：
potato spindle tuber viroid;;RT-PCR;;sequence analysis
摘  要：
The whole gene of potato spindle tuber viroid(Potato spindle tuber viroid, PSTVd), named PSTVd-IM, which was isolated from the diseased potato from Inner Mongolia, was cloned and sequenced in this research. The results showed that it had 359 bp and its sequence was the same as the isolate EF044305.1 from Russia. The phylogenetic tree of PSTVd isolate was built and analyzed based on the full-length nucleotide sequence of isolates from NCBI by MEGA5.1, which showed relativeness of PSTVd-IM with the isolates published. At the same time, homology analysis and second structure analysis of PSTVd-IM with different pathogenic PSTVd strains(severe strain U23058.1, intermediate strain AY937179.1 and weak strain M14814.1) were done to show the difference among them. This research would lay the foundation for studying the pathogenic mechanism.

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