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Position: Home > Articles > High Performance Liquid Chromatography Determination of Abamectin Residues in Dairy Products and Feed Journal of Dairy Science and Technology 2017,40 (1) 9-12

高效液相色谱法测定乳品与饲料中阿维菌素类药物残留

作  者:
岳虹;刘洋;赵贞;刘丽君;李翠枝;邵建波
单  位:
内蒙古伊利实业集团股份有限公司
关键词:
埃普利诺菌素;阿维菌素;多拉菌素;伊维菌素;三氟乙酸酐;N-甲基咪唑
摘  要:
建立乳品与饲料中阿维菌素类药物(埃普利诺菌素、阿维菌素、多拉菌素和伊维菌素)残留液相色谱的测定方法。乙腈为提取剂,tC_(18)固相萃取小柱净化,三氟乙酸酐和N-甲基咪唑柱前衍生,C_(18)柱分离,甲醇-乙腈的混合溶剂与水梯度洗脱,液相色谱仪荧光检测器检测,外标法定量。每1000 g乳粉(或每1000 mL液态乳)中阿维菌素添加量为1.5μg时,回收率大于95%。本方法液态乳、酸乳、乳粉的定量限为2μg/kg,饲料的定量限为10μg/kg。该方法具有结果准确、操作简便、重复性好等优点,可用于乳品和饲料中阿维菌素类药物残留的检测。
译  名:
High Performance Liquid Chromatography Determination of Abamectin Residues in Dairy Products and Feed
作  者:
YUE Hong;LIU Yang;ZHAO Zhen;LIU Lijun;LI Cuizhi;SHAO Jianbo;Inner Mongolia Yili Industrial Group Co.Ltd.;
关键词:
eprinomectin;;abamectin;;doramectin;;ivermectin;;trifluoroacetic anhydride;;N-methyl imidazole
摘  要:
A method for the deterrmnation of abamectin residues in dairy products and feed by using high performance liquid chromatography(HPLC) with fluorescence detection after precolumn derivation with trifluoroacetic anhydride and N-methyl imidazole was developed.Abamectin residues were extracted with acetonitrile,cleaned up on a tC_(18) solid phase extraction cartridge.After precolumn derivatization,the separation was performed on C_(18) column by gradient elution with methano-acetonitrile-water as mobile phase.The analyte were detected with a fluorescence detector and quantified by an external standard method.The recovery of abamectins spiked into milk powder at 1.5 μg/1 000 g(or 1 000 mL for milk) was higher than 90%.The limit of quantitation was 2 μg/kg for dairy products and 10.0 μg/kg for feed.This method proved to be accurate,simple reproducible,and suitable for the analysis of abamectin residues in dairy products and feed.

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