Qiang Meng;Yueyao Li;Yuan Yao;Shaowen Wu;Kun Shi;Shuwen Li

Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Yangling, Chin; Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, China; Viti-viniculture Engineering Technology Center of State Forestry and Grassland Administration, Yangling, China;College of Enology, Northwest A&F University, Yangling, China;Northwest A&F University

Acid stress;Cell viability;Lactiplantibacillus plantarum;Membrane lipids;Methionine;Transcription leve

This paper reported a wine-derived lactic acid bacterium, Lactiplantibacillus plantarum XJ25, which exhibited higher cell viability under acid stress upon methionine supplementation. Cellular morphology and the composition of the cytomembrane phospholipids revealed a more solid membrane architecture presented in the acid-stressed cells treated with methionine supplementation. Transcriptional analysis showed L. plantarum XJ25 reduced methionine transport and homocysteine biosynthesis under acid stress. Subsequent overexpression assays proved that methionine supplementation could alleviate the cell toxicity from homocysteine accumulation under acid stress. Finally, L. plantarum XJ25 employed energy allocation strategy to response environmental changes by balancing the uptake methionine and adjusting saturated fatty acids (SFAs) in membrane. These data support a novel mechanism of acid resistance involving methionine utilization and cellular energy distribution in LAB and provide crucial theoretical clues for the mechanisms of acid resistance in other bacteria.