中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 脑多头蚴重组蛋白rTm16和rTm-GST的免疫保护效果分析 畜牧兽医学报 2018 (12) 2707-2714
Position: Home > Articles > Immune Protective Effect Analysis of Recombinant Protein rTm16 and rTm-GST from Coenurus cerebralis Chinese Journal of Animal and Veterinary Sciences 2018 (12) 2707-2714

脑多头蚴重组蛋白rTm16和rTm-GST的免疫保护效果分析

作  者：
郭承;万洁;杨应东;文建国;刘俞辰;古小彬;谢跃;杨光友
单  位：
四川农业大学动物医学院;攀枝花市农林科学研究院
关键词：
脑多头蚴;免疫评价;Tm16;GST;山羊
摘  要：
为探究多头带绦虫16 k Da蛋白和GST蛋白对山羊脑多头蚴病的免疫保护效果,44只攀枝花本地黑山羊随机分为4组(rTm16疫苗组、rTm-GST疫苗组、rTm16+rTm-GST疫苗组以及对照组),皮下免疫三次,首免后4周进行2免,2免后6个月进行3免,定时采血并分离血清,使用间接ELISA方法检测血清中特异性Ig G水平;末次免疫后2周,各组山羊每只口服攻击多头带绦虫的虫卵约5 500个,感染105 d后剖检记录脑多头蚴包囊数量,计算减囊率。结果显示:相较于对照组,rTm16组、rTm-GST组、rTm16+rTm-GST组分别获得50%、62. 5%、87. 5%的减囊率,rTm16+rTm-GST免疫组山羊保护效果最好(P=0. 005)。ELISA检测结果显示,攻虫以前,rTm-GST组特异性抗体水平均显著低于rTm16组(P <0. 05),而联合免疫组中rTm-GST所诱导的特异性抗体与rTm16所诱导的特异性抗体水平差异不显著(P>0. 05)。rTm16与rTm-GST的配合使用能够诱导山羊产生较高抗体水平,从而获得较显著的免疫保护力。
译  名：
Immune Protective Effect Analysis of Recombinant Protein rTm16 and rTm-GST from Coenurus cerebralis
作  者：
GUO Cheng;WAN Jie;YANG Ying-dong;WEN Jian-guo;LIU Yu-chen;GU Xiao-bin;XIE Yue;YANG Guang-you;College of Veterinary Medicine,Sichuan Agricultural University;Panzhihua Academy of Agricultural and Forestry Sciences;
单  位：
GUO Cheng%WAN Jie%YANG Ying-dong%WEN Jian-guo%LIU Yu-chen%GU Xiao-bin%XIE Yue%YANG Guang-you%College of Veterinary Medicine,Sichuan Agricultural University%Panzhihua Academy of Agricultural and Forestry Sciences
关键词：
Coenurus cerebralis;;immune evaluation;;Tm16;;GST;;goat
摘  要：
To explore the immune protective effect of recombinant Tm16 and GST proteins against Coenurus cerebralis in goats,forty-four goats were divided into four groups,including rTm16 group,rTm-GST group,rTm16 + rTm-GST group and control group,which were immunized 3 times by subcutaneous injection,respectively. Second vaccination was performed 4 weeks after the first vaccination,and 6 months later the last vaccination was performed. The serum was regularly collected from each goat,and the specific IgG response to each group was measured by indirect ELISA. Two weeks after the final vaccination,each goat was orally challenged with 5 500 eggs of Taenia multiceps. One hundred and five days post infection all goats were slaughtered,the cysts in brain and muscle were collected and counted. Result were as follows: Compared with the control group,the cysts of goat in group rTm16,rTm-GST and rTm16 +rTm-GST reduced by 50%,62. 5% and 87. 5%,respectively. Combined immunization of rTm16 with rTm-GST induced significant protection( P < 0. 05) against challenge of T. multiceps in goats. Result ofi ELISA showed that the specific IgG response against rTm-GST was significantly weaker than that against rTm16( P < 0. 05). However,the specific IgG response against rTm-GST was not significant compared with that against rTm16 in combined group( P > 0. 05). This revealed that the combined use of rTm16 and rTm-GST could induce higher antibody levels in goats,thus obtaining significant protective immunity.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46