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Position: Home > Articles > Optimization of a reaction system of sequence related amplified polymorphism and segregation of polymorphic loci in an F2 population of rice. Rice Science 2008,15 (2) 95-100

Optimization of a reaction system of sequence related amplified polymorphism and segregation of polymorphic loci in an F2 population of rice.

作  者:
Jiang ShuKun;Zhong Ming;Zhang XiJuan;Zhang Li;Xu ZhengJin
单  位:
Key Laboratory of Rice Breeding of Liaoning Province; Shenyang 110161; China;Key Laboratory of Agricultural Biotechnology of Liaoning Province; Shenyang Agricultural University
关键词:
crosses;crossing;loci;polymerase chain reaction;populations;rice;Oryza;Oryza sativa
摘  要:
An effective PCR protocol for detecting the sequence related amplified polymorphism (SRAP) in rice was developed. One hundred and ten pairs of SRAP primers were used for segregation analysis in an F2 population derived from a cross between Shennong 606 and Lijiangxintuanheigu. Among the 110 primer pairs, 35 pairs generated 143 polymorphic bands with an average of 4.09 polymorphic bands per primer pair, and 24 pairs (16.78%) showed the genetic distortion (P<0.05). Of the 24 primer pairs, 12 pairs deviated toward the male parent Shennong 606 and 11 pairs toward the female parent Lijiangxintuanheigu, only one toward heterozygote. It was found that the segregation distortion might be caused by the joint gametic and zygotic effects.

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