Specific PCR detection of Colletotrichum fructicola causing anthracnose on plum trees

Plum anthracnose%Colletotrichum fructicola%species-specific primer%PCR detection

Colletotrichum fructicola,belonging to the C.gloeosporioides species complex,is the dominant pathogenic species causing anthracnose on plum trees.In this study,multiple alignment of ApMat sequences of 36 strains in the C.gloeosporioides complex was performed,a pair of C.fructicola-specific primers:F2(5'-CGTGACCCAGGAGGCGACCACGCATCTGT-3')and R2(5'-GGTGATCTCTCCTAGCGTTCGTACGTCTA-AT-3')was designed based on the specific sequences of the pathogen,and a PCR detection method was develo-ped accordingly.The results showed that a specific target amplicon of 132 bp could only be produced by C.fructicola with the primer pair F2/R2,with a detection sensitivity of 100 fg·μL-1.Using this PCR detection system,the target pathogen C.fructicola can be quickly detected from the leaves of plum trees artificially inocu-lated with C.fructicola HN47-2 strain and the leaves of plum trees naturally infected with C.fructicola.The C.fructicola-specific PCR detection method established in this study provides an important technical support for early diagnosis,dynamic monitoring and accurate prevention and control of plum anthracnose caused by C.fructicola in the field.