作 者：
朱天华;陆锦明;孙清;王水燕;姚德彪;顾建平;汤桂钧
单 位：
上海旭东园艺有限公司;上海市闵行区农业良种繁育场;上海市闵行区农业科学研究所
摘 要：
以百合科十二卷属植物的当年抽生的花枝为外植体,经洗涤剂清洗后置流水下冲洗4h。无菌条件下将花枝消毒杀菌,无菌水冲洗3遍后切成1～1.5cm的茎段,接种至发生培养基上诱导成无菌系;筛选出合适的发生培养基配方,以MS+2mg/L BA+0.2mg/L NAA较好。继代增值配方以MS+1～2mg/L BA+0.1～0.2mg/L NAA较好,增值率可达1∶3～4。生根培养的配方为1/2MS+0.2mg/L NAA,生根率可达96%左右。试管苗炼苗成活率高达98%以上。
译 名：
Study on the Culture of Haworthia fasciata in vitro
作 者：
ZHU Tian-hua1,LU Jin-ming1,SUN Qing1,WANG Shui-yan1, YAO De-biao2,GU Jian-ping2,TANG Gui-jun3(1.Shanghai Minhang Farm for Agricultural Breeding Farm,Shanghai 201109,China; 2.Shanghai Xudong Horticulture Co.,Ltd,Shanghai 201112,China; 3.Shanghai Minhang District Institute for Agricultural Science,Shanghai 201109,China)
关键词：
Haworthia fasciata;in vitro culture;test-tube seedling
摘 要：
The explants from branches of Haworthia fasciata of liliaceae were cut and flushed with tap water for 4 hours after being washed with detergent.The explants were sterilized in clean bench,rinsed three times with sterile water,cut into 1-1.5 cm of pieces,and then inoculated on regeneration medium to induce sterile lines in turn.The selected optimal regeneration medium,subculture medium and rooting medium were MS+BA2 mg/L+NAA0.2 mg/L,MS+BA1-2 mg+NAA0.1-0.2 mg/ with an amplification ratio of 1:3-4,and 1/2MS+NAA0.2 mg/L with a rooting efficiency rate of 96%,respectively.The successful transplanting rate of test-tube seedling was more than 98%.
