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Position: Home > Articles > Optimization of Complex Enzymatic Extraction of Flavone From Flammulina velutiper(Fr.) Sing Hubei Agricultural Sciences 2015 (14) 3482-3484+3488

复合酶法提取金针菇总黄酮的优化

作  者:
王广慧;魏雅冬;于德涵
单  位:
绥化学院食品与制药工程学院
关键词:
复合酶法;金针菇[Flammulina velutiper(Fr.)Sing];总黄酮;提取
摘  要:
为了研究采用复合酶法从金针菇[Flammulina velutiper(Fr.)Sing]中提取总黄酮的最适条件,通过单因素试验和正交试验探讨了料液比、酶反应时间、酶反应温度、酶反应p H对金针菇总黄酮提取率的影响,并以金针菇总黄酮提取率为评价指标,优化提取工艺。结果表明,复合酶法提取金针菇总黄酮的最适条件为料液比1∶20(g∶m L),酶反应时间80 min,酶反应温度60℃,酶反应p H 5,在此条件下金针菇总黄酮的提取率为4.76%。
译  名:
Optimization of Complex Enzymatic Extraction of Flavone From Flammulina velutiper(Fr.) Sing
作  者:
WANG Guang-hui;WEI Ya-dong;YU De-han;Department of Pharmacy Chenical Engineering, Suihua University;
关键词:
complex enzyme;;Flammulina velutiper(Fr.) Sing;;total flavone;;extraction
摘  要:
In this paper, the optimal conditions of complex enzymatic extraction of flavone from Flammulina velutiper(Fr.)Sing were studied. Through the single factor and orthogonal experiments, the influences of solid-liquid ratio, enzymatic reaction time, enzymatic reaction temperature and enzymatic reaction p H on the extraction rate of flavone were discussed, and the extraction process was optimized. The experimental results showed that the optimum conditions were as follows : the solidliquid ratio of 1:20(g / m L), enzymatic reaction time of 80 min, enzymatic reaction temperature of 60 ℃, enzymatic reaction p H of 5. Under this condition, flavone extraction rate was 4.76%.

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