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Position: Home > Articles > Comparative Mapping of the Dwarf Gene Bu from Tropical Pumpkin(Cucurbita moschata Duchesne) Acta Horticulturae Sinica 2011,38 (1) 95-100

南瓜矮生基因Bu的比较定位

作  者:
王深浩;李海真;张忠华;贺俊;贾长才;张帆;黄三文
单  位:
北京市农林科学院蔬菜研究中心;中国农业科学院蔬菜花卉研究所
关键词:
南瓜;矮生基因;比较基因组学;分子标记
摘  要:
以中国南瓜矮生突变体为供体亲本,以印度蔓生南瓜为轮回亲本,构建了BC6F2分离群体。利用黄瓜基因组序列,将南瓜矮生基因Bu比较定位至黄瓜5号染色体,并开发了一个新的PCR标记IF3629,该标记与矮生基因Bu连锁遗传距离为1.0cM。该标记不仅可以用于分子标记辅助选择育种,而且为Bu基因的克隆奠定了基础。
译  名:
Comparative Mapping of the Dwarf Gene Bu from Tropical Pumpkin(Cucurbita moschata Duchesne)
作  者:
WANG Shen-hao1,LI Hai-zhen2,ZHANG Zhong-hua1,HE Jun1,JIA Chang-cai2,ZHANG Fan2,and HUANG San-wen1,(1Key Laboratory of Horticultural Crops Genetic Improvement of Ministry of Agriculture,Sino-Dutch Joint Lab of Horticultural Genomics Technology,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
关键词:
squash;dwarf gene;comparative genomics;molecular marker
摘  要:
A segregating BC6F2 population was developed using a dwarf mutant of Cucurbita moschata Duch.as donor and the vine C.maxima Duch.'Mengri'as recurrent parent.Using the segregating population,the gene Bu was comparatively mapped on chromosome 5 of cucumber.An intron-flanking marker IF3629 was found to be tightly linked to the Bu gene,with a genetic distance of 1.0 cM.The marker IF3629 will not only be useful in markers-assisted selection for dwarf plant,but also provide a starting point toward positional isolation of the dwarf Bu gene in squash.

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