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Position: Home > Articles > Phytochrome B Positively Regulates Chlorophyll Biosynthesis and Chloroplast Development in Rice Chinese Journal of Rice Science 2012,26 (6) 637-642

光敏色素B正调控水稻叶绿素合成和叶绿体的发育

作  者:
赵杰;周晋军;顾建伟;钱凤芹;谢先芝
单  位:
山东省农业科学院高新技术研究中心;山东师范大学生命科学学院
关键词:
水稻;光敏色素;叶绿素合成;PORA基因;叶绿体发育
摘  要:
水稻光敏色素基因家族包括3个成员,PHYA、PHYB和PHYC,它们在调控水稻去黄化、花期和育性等光形态建成中具有重要作用。我们比较了白光和红光条件下野生型、phyA、phyB和phyAphyB突变体中叶绿素含量。结果表明,phyB感受红光正调控水稻叶绿素合成,phyA的作用仅仅在phyB功能缺陷时才能表现出来。叶绿素合成相关基因表达模式分析结果表明,水稻光敏色素介导的光信号主要通过调控原叶绿素酸酯氧化还原酶基因(protochlorophyll oxidoreduc-tase A,PORA)的表达而影响叶绿素的合成。同时,还分析了phyB介导的红光信号对叶绿体发育的影响,结果表明,phyB介导的红光信号在调控叶绿体数目、基粒数目及叶绿体膜发育中具有重要作用。
译  名:
Phytochrome B Positively Regulates Chlorophyll Biosynthesis and Chloroplast Development in Rice
作  者:
ZHAO Jie1,2,ZHOU Jin-jun2,GU Jian-wei2,QIAN Feng-qin2,XIE Xian-zhi1,2,*(1College of Life Sciences,Shandong Normal University,Jinan 250014,China;2High-Tech Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China;)
关键词:
rice;phytochrome;chlorophyll biosynthesis;PORA;chloroplast development
摘  要:
Phytochrome family in rice is composed of three members,PHYA,PHYB,and PHYC.Through characterizing the phytochrome mutants and wild type(WT) in terms of photomorphogenesis,roles of individual phytochromes have been preliminarily explored in regulating rice de-etiolation,flowering time and fertility.We compared the chlorophyll contents of wild type and phyA,phyB and phyAphyB mutants grown either in white light(W) or in red light(R).The results suggest that phyB perceives red light to positively regulate chlorophyll biosynthesis,while the role of phyA can be detected only in the phyB-deficient mutant.Analyses on the expression levels of genes involved in chlorophyll biosynthesis revealed that phytochromes affected chlorophyll biosynthesis by regulating protochlorophyll oxidoreductase A(PORA) expression.The role of phyB in chloroplast development was analyzed.The results suggest that phyB perceive red light to regulate chloroplast development by affecting the number of chloroplasts and grana,as well as the chloroplast membrane system.

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