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Position: Home > Articles > Study on molecular genetic polymorphism of Anopheles sinensis populations in China Acta Entomologica Sinica 2001,44 (1) 33-39

我国中华按蚊群体分子遗传多态研究

作  者:
雅军;瞿逢伊;徐建农;郑哲民
单  位:
陕西师范大学生命科学学院;第二军医大学基础部寄生虫学教研室
关键词:
中华按蚊;群体;分子遗传多态
摘  要:
利用RAPD PCR技术研究了采自我国 9省 10个代表点的中华按蚊Anophelessinensis自然群体的遗传多态现象 ,根据 2 3个RAPD等位基因位点进行了分析。结果显示 :①多态位点比例为6 8 2 %~ 86 4 % ,期望平均杂合度为 0 2 4 9~ 0 348,说明中华按蚊群体具广泛的遗传多态现象 ;②利用 3种方法计算Fst和θ,平均值为 0 0 6 9~ 0 111,相应的迁移率Nm为 2 0~ 3 4 ,表明基因流水平较低 ;③中华按蚊各自然群体间的遗传一致性达 0 8795~ 0 9973,平均遗传距离为0 0 4 1± 0 0 33,属种内变异范围。聚类分析显示 ,群体间遗传距离与地理位置无对应关系。
译  名:
Study on molecular genetic polymorphism of Anopheles sinensis populations in China
作  者:
MA Ya jun 1, QU Feng yi 1, XU Jian nong 1, ZHENG Zhe min 2 (1.Department of Parasitology, Second Military Medical University, Shanghai\ 200433,China; 2.College of Life Sciences, Shaanxi Normal University, Xian\ 710062,China)
关键词:
Anopheles sinensis; population; molecular genetic polymorphism
摘  要:
Samples of Anopheles sinensis from 10 collection sites in 9 provinces of China were analyzed for genetic polymorphism using RAPD PCR. Twenty three RAPD loci were studied and the results were as follows:① The percentage of polymorphic loci was 68 2%~86 4%, and the expected mean heterozygosity was 0 249~0 348, which indicated extensive genetic polymorphism in the natural populations of An.sinensis. ② The \%Fst\% and θ were caculated using 3 measures. Mean values were 0 069~0 111 with corresponding migrating rate of 2 0~3 4. It indicated that the level of gene flow was low. ③The genetic identity among the natural populations was 0 8795~0 9973 with the mean genetic distance of 0 041±0 033. The data are within the range of intraspecific variation. Cluster analysis revealed that the genetic distance did not relate to the collection sites.

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