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Position: Home > Articles > Evaluation of Three Serological Tests for Brucellosis in Cattle Using Bayesian Analysis Chinese Journal of Animal and Veterinary Sciences 2010,41 (4) 449-453

3种奶牛布鲁氏菌病血清学检测方法的贝叶斯评估

作  者:
刘晓民;杨华;高明春;李彦伟;王君伟
单  位:
东北农业大学动物医学学院
关键词:
布鲁氏菌病;虎红平板凝集试验;间接酶联免疫吸附试验;荧光偏振分析法;贝叶斯分析
摘  要:
在无"金标准"的情况下,为评估虎红平板凝集试验(RBT)、荧光偏振分析法(FPA)和间接酶联免疫吸附试验(I-ELISA)用于奶牛布鲁氏菌病的抗体检测能力,作者分别以RBT、FPA、I-ELISA3种方法对采自某奶牛场的血清样品进行检测,通过贝叶斯分析法对检测结果进行分析,研究3种方法检测奶牛布鲁氏菌血清抗体的敏感性(Se)和特异性(Sp)。结果显示,RBT、FPA、I-ELISA3种检测方法的敏感性(Se)分别为91.81%、95.92%和95.98%;特异性(Sp)分别为98.80%、86.54%和98.65%。本研究表明,3种血清学检测方法中以I-ELISA的检测能力最好。
译  名:
Evaluation of Three Serological Tests for Brucellosis in Cattle Using Bayesian Analysis
作  者:
LIU Xiao-min,YANG Hua,GAO Ming-chun,LI Yan-wei,WANG Jun-wei(College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China)
关键词:
Brucellosis;RBT;I-ELISA;FPA;Bayesian analysis
摘  要:
The objective of our study was to evaluate the diagnostic performance of three serological tests for brucellosis.Serological test results were used to compare the performance of Rose Bengal Test(RBT),indirect ELISA(I-ELISA)and Fluorescence Polarisation Assay(FPA)in diagnosing brucellosis in cattle.Their performance was evaluated using Bayesian analysis which allows the sensitivity(Se)and specificity(Sp)to be estimated in the absence of a gold standard.The sensitivity of RBT,FPA and I-ELISA were 91.81%,95.92% and 95.98%,respectively;and specificity of RBT,FPA and I-ELISA were 98.80%,86.54% and 98.65%,respectively.The results demonstrated that the I-ELISA performing well in diagnosing brucellosis in cattle.

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