中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 虾夷扇贝三倍体诱导与培育技术的研究 中国水产科学 2001,8 (1) 18-22
Position: Home > Articles > Induced triploidy in scallop Patinopecten yessoensis and the larval cultivation Journal of Fishery Sciences of China 2001,8 (1) 18-22

虾夷扇贝三倍体诱导与培育技术的研究

作  者：
常亚青;相建海;张国范;王子臣;宋坚
单  位：
中国科学院海洋研究所;大连水产学院
关键词：
虾夷扇贝;三倍体诱导;6-DMAP;幼体培育
摘  要：
:取繁殖期虾夷扇贝 (Patinopectenyessoensis)亲体 ,采用阴干、流水和升温海水催产以及小水体、高密度、雌雄分池排放、人工授精的方法 ,可获得批量受精卵。分别使用浓度为 0 .0 5,0 .10 ,0 .15,0 .2 0 ,0 .30 ,0 .50和 0 .70mmol/L的 6 -DMAP诱导虾夷扇贝三倍体 ,实验水温分别为 12℃和 14℃。结果表明 ,水温12℃ ,6 -DMAP 0 .0 5mmol/L时 ,在授精后 6 7min处理胚胎 4 5min诱导三倍体效果较好 ,三倍体率达 83.3% ,面盘幼虫相对孵化率达 6 9.5% ;水温 12℃和 14℃时 ,6 -DMAP可诱导 17.3%～ 10 0 .0 %的三倍体面盘幼虫 ;6 -DMAP处理组胚胎发育至面盘幼虫的时间比对照组慢 ,故应推迟 1d选优 ;处理组幼虫初始壳长、壳高及日生长速度皆高于对照组 ;6 -DMAP的残留会影响处理组幼虫的成活率 ,用 0 .2ml/L的DMSO海水浸洗处理可减轻 6 -DMAP的副作用。
译  名：
Induced triploidy in scallop Patinopecten yessoensis and the larval cultivation
作  者：
CHANG Ya qing 1, XIANG Jian hai 1, ZHANG Guo fan 2, WANG Zi chen 2, SONG Jian 2 (1.Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China; 2.Dalian Fisheries University, Dalian 116023, China)
关键词：
Patinopecten yessoensis; triploid induction; 6-DMAP; larval cultivation
摘  要：
Triploid was induced in Patinopecten yessoensis using 6-dimethylaminopurine (6-DMAP) at different concentration levels: 0.05, 0.10, 0.15, 0.20, 0.30, 0.50 and 0.70 mmol/L. The water temperature was designed at 12℃ and 14℃, respectively. At both temperatures the triploidy larva rate got to 17.3%～100%. An optimum effect could be got at water temperature 12℃ and 6-DMAP concentration 0.05 mmol/L when the treatment began at 67 th minute after the fertilization and went on for 45 min that the triploidy maxima was 83.3% and the corresponding hatching rate of veliger larvae was 69.5%. The residue of 6-DMAP can affect the survival of triploidy larvae but the side effect can be lightened by using of DMSO at 2 ml/L sea water for 5 d. The embryos in 6-DMAP-treated groups grow more slowly than those in control during their developing into veligers, so the treated larvae should be selected 1 day later for cultivation compared with the control. The initial shell length and height, as well as the daily growth rate of the larvae in treated groups are all higher than those in control.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46