中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 龙胆石斑鱼源美人鱼发光杆菌的生物学特性与系统发育学分析 渔业科学进展 2009,30 (3) 38-43
Position: Home > Articles > Biological characterization and phylogenetic analysis of Photobacterium damselae subsp.damselae from diseased Epinephelus lanceolatus L. Progress in Fishery Sciences 2009,30 (3) 38-43

龙胆石斑鱼源美人鱼发光杆菌的生物学特性与系统发育学分析

作  者：
张晓君;秦国民;陈翠珍;房海;阎斌伦
单  位：
河北科技师范学院动物科学系;淮海工学院海洋学院
关键词：
龙胆石斑鱼;美人鱼发光杆菌美人鱼亚种;生物学特性;系统发育学
摘  要：
对从1尾病死的观赏用龙胆石斑鱼Epinephelus lanceolatus L.中分离到的细菌,进行了形态特征、理化特性和对抗菌类药物的敏感性等较系统的表观生物学性状鉴定。同时,测定了16S rRNA基因序列、分析了相关细菌相应序列的同源性、构建了系统发生树。结果表明,供试两株纯培养菌(编号:HQ061227-1、HQ061227-2)为发光杆菌属Photobacterium(Beijerinck 1889)的美人鱼发光杆菌美人鱼亚种P.damselae subsp.damselae(Love et al.1982;Smith et al.1991),用HQ061227-1株作为代表菌株的16S rRNA基因序列长度(不包括引物结合区)为1469bp(GenBank登录号:EF635307),与GenBank数据库中美人鱼发光杆菌美人鱼亚种的同源性在99%。药敏试验结果显示,对供试37种抗菌药物中的青霉素G等4种耐药,对头孢唑啉等32种敏感,对氨苄青霉素低敏。
译  名：
Biological characterization and phylogenetic analysis of Photobacterium damselae subsp.damselae from diseased Epinephelus lanceolatus L.
作  者：
ZHANG Xiao-jun1 QIN Guo-min1CHEN Cui-zhen2 FANG Hai2 YAN Bin-lun1(1College of Ocean, Key Laboratory of Oceanic Biotechnology of Jiangsu, Huaihai Institute of Technology, Lianyungang 222005)(2Department of Animal Science, Hebei Normal University of Science and Technology,Qinhuangdao 066600)
关键词：
Epinephelus lanceolatus L. Photobacterium damselae subsp. damselaeBiological characterization Phylogenetic analysis
摘  要：
The biological characteristics and phylogenetic analysis of bacteria isolated from Epinephelus lanceolatus L. were studied, including morphological characteristics, physiological and biochemical characteristics. In addition, the 16S rRNA gene were partially sequenced and compared with sequences registered in databases; and molecular phylogenetic trees were constructed. The results showed that the two pure strains (HQ061227-1、HQ061227-2)belonged to P. damselae subsp. damselae (Love et al. 1982; Smith et al. 1991) of Photobacterium (Beijerinck 1889 ), the sequenced 16S rRNA gene of strain HQ061227-1(accession No. EF635307) is 1 469bp in length, and exhibited high similarity (99%) with the 16S rRNA gene of P. damselae from GenBank database. Antibiotic sensitivity using 37 antimicrobial agents showed that the isolates were sensitive to 32 agents including cefazolin, resistant to 4 agents including penicillin G, and slightly sensitive to ampicillin.

相似文章

导出引用
计量
文章访问数: 19
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46