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Xenorhabdus poinarii 5-5B杀虫毒素的分离纯化与活性测定

作  者:
王欢;丛斌;董辉;钱海涛
单  位:
沈阳农业大学生物科学技术学院;沈阳农业大学植物保护学院
关键词:
昆虫病原线虫共生菌;杀虫毒素;胃毒活性
摘  要:
首次从全国采集的土样,分离并筛选出高毒力共生菌菌株Xenorhabdus poinarii 5-5B,首次对Xenorhabdus poinarii的杀虫毒素进行分离纯化,通过硫酸铵沉淀法对其杀虫毒素进行分离后,再利用DEAE-52层析柱进行了纯化,共得到7个峰,各峰的回收产物进行活性测定(干粉以5 μg/L溶于去离子水)结果表明,各峰产物对亚洲玉米螟、黄粉虫、小菜蛾、黏虫和甜菜夜蛾均具有较高的胃毒活性。
译  名:
Isolation and Purification of Insecticidal Proteins from Xenorhabdus poinarii 5-5B
作  者:
WANG Huan a ,CONG Bin b ,DONG Hui b ,QIAN Hai-tao b (Shenyang Agricultural University a.College of Biological Science and Technology;b.College of Plant Protection,Shenyang 110161,China)
关键词:
entomopathogenic nematode symbiotic bacteria;insecticidal toxins;oral toxicity
摘  要:
Highly virulent strain(Xenorhabdus poinarii 5-5B)of symbiotic bacteria were isolated and selected from entomopathogenic nematodes which gathered in China.The seven insecticidal toxins of X.pionarii 5 were pured by DEAE-52 chromatography.Bioassay results showed all toxins had highly virulence to Ostrinia furnacalis,Tenebrio molitor,Plutella xylostella,Mythimna separata,Latphygma exigua.

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