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碱法水解羽毛蛋白的工艺条件研究

作  者:
尹国强;崔英德;黎新明;贾振宇;廖列文
单  位:
西北工业大学材料科学与工程学院;仲恺农业技术学院绿色化工研究所
关键词:
羽毛;角蛋白;水解;最佳条件;蛋白质
摘  要:
利用亚硫酸氢钠溶液对羽毛进行预处理,采用氢氧化钠溶液水解羽毛制得可溶性羽毛蛋白。考察了预处理剂的用量、碱液浓度、液固比、水解时间、水解温度等因素对羽毛水解所得可溶性蛋白收率的影响。得到水解最佳条件:ω(亚硫酸氢钠):ω(羽毛粉)=30:100、ω(氢氧化钠)=0.4%水溶液、ω(氢氧化钠深液):ω(羽毛粉)=15:1、反应温度90℃、反应时间2h。在此条件下制得的可溶性羽毛蛋白的收率达65.7%。
译  名:
Study on the Optimum Conditions of the Hydrolyzation of Feather Keratins by Alkaline
作  者:
YIN Guo-qiang, CUI Ying-de, LI Xin-ming, JIA Zhen-yu, LIAO lie-wen (College of Materials Science and Engineering, North Western Polytechnical University, Xi'an 710072, China; Institute of Green Chemical Engineering, Zhongkai University of Agricultural and Technology, Guangzhou 510225,China)
关键词:
feather; keratin; hydrolysis; optimum condition; protein
摘  要:
A new method of extracting soluble protein from feather was introduced in the paper. Feather was preprocessed with sodium bisulfite and then hydrolysised by sodium hydroxide. After investigating the eωct factors such as amounts of sodium bisulfite, alkaline solution concentration, ratio of liquid to solid, hydrolytic temperature and hydrolytic time, the optimum hydrolytic conditions were obtained as follows: ω ( sodium bisulfite); ω ( feather)=30:100, alkaline solution concentration 0. 4%, ω(liquid); ω(feather)=15:1, hydrolytic temperature 90℃ and hydrolytic time 2 h. Under these conditions, the recovery ratio of the soluble feather protein was 65.7%.

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