作 者：
SUN Hai-cheng;Lü Xiao-nan;TONG Guang-xiang;YIN Jia-sheng;XUE Shu-qun;ZHANG Li-na;HAN Ying;College of Animal Science and Technology, Northeast Agricultural University;Beijing Aquatic Product Technology Promotion Department;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences;
单 位：
SUN Hai-cheng%Lü Xiao-nan%TONG Guang-xiang%YIN Jia-sheng%XUE Shu-qun%ZHANG Li-na%HAN Ying%College of Animal Science and Technology, Northeast Agricultural University%Beijing Aquatic Product Technology Promotion Department%Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences
关键词：
Oxygymnocypris stewarti;;Real-time quantitative PCR;;reference gene
摘 要：
The expression of 7 candidate reference genes including GAPDH, HPRT1, RPL13, RPL19, RPL13a, SDHA and ACTB was comparatively investigated in liver, spleen, head kidney, midkidney, metanephros, gill, foregut, midgut, hindgut, heart, eyes, pituitary, blood, brain, skin, red muscle, white muscle and gonad of Stewart's naked high-Asian-carp Oxygymnocypris stewarti by Real-time quantitative PCR to screen out stable reference genes for Real-time quantitative PCR analysis in Stewart's naked high-Asian-carp, and GeNorm, NormFinder and BestKeeper software were used to analyze the stability of the candidate internal reference genes. The GeNorm analysis revealed that the descending order of the average expression stability value M in 7 candidate genes was expressed as RPL13=RPL13a
