Comparative Study of Myocardial Infarction Models in Two Kinds of Rat

LIU Zhen1,LIU Ling-ling1,YANG Ting-tong2(1.The Central Hospital of Xinxiang Medical Univisity,Xinxiang,Henan,453000,China;2.Morphology Lab,Xinxiang Medical Univisity,Xinxiang,Henan,453003,China)

coronary artery ligation method;medicament injection method;isoprenaline;myocardial infarction;rat

Comparison of the myocardial infarction models were produced by the coronary artery ligation method and drug injection method were used to explore the advantages and disadvantages and applications of the two models.The 80 SD rats were randomly assigned to the coronary artery ligation groups and drug injection groups.The rats in coronary artery ligation group were endotracheal intubation and were connected with small animal ventilator,and then the heart was exposed,and the left anterior descending coronary artery in the upper 1/3 was ligated.The rats in drug injection group injected isoproterenol and continuous injection of two days.After two groups of rats were treated,the rats were killed respectively in 24h,48h,72h,1 w,2 w,and made heart TTC staining,HE staining and IHC for histopathological observation and analysis.The TTC staining showed:The rat models of myocardial infarction made by the coronary artery ligation and drug injection group appeared sallow region of myocardial infarction.The region of myocardial infarction in the coronary artery ligation groups mostly showed larger infarct range.The region of myocardial infarction in the drug injection groups mostly showed more smaller range and scattered.The HE staining showed:The myocardial tissue of two kinds of methods rats all emerged coagulation necrosis,inflammatory cell invasion,then,there was granulation tissue in myocardial infarction,the scar formea finally.In the coronary ligation groups,the myocardial cells mostly were complete infarction,and the size of infarct area was positively correlated with the degree of ligation site.The myocardial cells in the drug injection groups occasionally seen remnants of myocardial cells within the infarcted area.The infarct area of drug injection groups mainly distributed to the left ventricular wall and septum,especially near the heart apexelse.The IHC showed:Both two groups have a strong expression to cardiac apoptosis-related gene P53 and Caspase-3.The number of death in coronary artery ligation rats was more than that of drug injection groups and compared with the coronary artery ligation method,the drug injection method was easier to master,without a lot of instruments and equipment.The coronary artery ligation method and drug injection method were both effective to copy the model of myocardial infarction,and had their own advantages and characteristics.We can reasonably use of two methods based on technical requirements and the practical needs in our scientific research work.