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当前位置: 首页 > 文章 > 冰温贮藏中南美白对虾特定腐败菌的分离鉴定及其腐败能力分析 食品工业科技 2016,37 (5) 171-176
Position: Home > Articles > Isolation,identification and spoilage ability analysis on the particular spoilage bacteria of penaeus vannamei in the ice temperature storage Science and Technology of Food Industry 2016,37 (5) 171-176

冰温贮藏中南美白对虾特定腐败菌的分离鉴定及其腐败能力分析

作  者：
谢丽丹;李蕾蕾;王素英;董世瑞
单  位：
天津市食品生物技术重点实验室天津商业大学生物技术与食品科学学院
关键词：
南美白对虾;冰温贮藏;腐败菌;分离鉴定;腐败能力
摘  要：
为了分离鉴定南美白对虾在冰温贮藏条件下的腐败菌,并分析其腐败能力的大小。通过冻结实验,确定南美白对虾的冰点,在0℃与冰点之间贮藏南美白对虾,从中分离腐败菌,进行形态学观察和分子生物学鉴定,将分离得到的腐败菌接种至灭菌虾汁中,通过产硫化氢,降解蛋白质,产挥发性盐基氮(TVB-N)的测定衡量腐败能力的大小。结果表明:从南美白对虾中分离得到12株腐败菌,分别是Shewanella hafniensis,Acinetobacter johnsonii,Planoccoccus citreus,Bacillus cereus,Acinetobacter beijerinckii,Enterobacter hormaechei,Arthrobacter bergeri,Bacillus licheniformis,未鉴定出的菌株X1和X10。在冰温贮藏条件下,导致南美白对虾腐败的优势腐败菌依次是Shewanella hafniensis、X1、Acinetobacter johnsonii和Acinetobacter beijerinckii。
译  名：
Isolation,identification and spoilage ability analysis on the particular spoilage bacteria of penaeus vannamei in the ice temperature storage
作  者：
XIE Li- dan;LI Lei- lei;WANG Su- ying;DONG Shi-rui;Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce;
关键词：
penaeus vannamei;;ice temperature storage;;dominant spoilage bacteria;;isolation and identification;;spoilage ability
摘  要：
In order to isolate and identify penaeus vannamei,s spoilage bacteria and measure their spoilage ability in ice temperature storage conditions,the freezing point of penaeus vannamei was determined based on freezing experiment and the spoilage bacteria were separated from penaeus vannamei which was stored under the temperature between 0 ℃ and the freezing point,the isolated microbes were identified by morphological observation and molecular biology methods.The separated spoilage bacteria were inoculated into sterilized shrimp juice,hydrogen sulfide,degradated proteins and volatile base nitrogen( TVB- N) were selected to evaluate the spoilage ability.The results showed that 12 strains of spoilage bacteria were isolated from penaeus vannamei,they were Shewanella hafniensis,Acinetobacter johnsonii,Planoccoccus citreus,Bacillus cereus,Acinetobacter beijerinckii,Enterobacter hormaechei,Arthrobacter bergeri,Bacillus licheniformis,of which X1 and X10 may be a potential new species or genus. In ice temperature storage conditions,dominant spoilage bacteria leading to the corruption of penaeus vannamei were Shewanella hafniensis,X1,Acinetobacter johnsonii and Acinetobacter beijerinckii.

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