Position: Home > Articles > Identification for Genetically Modified Rape RT73 Line with SYBR~ Green I Real Time PCR
Journal of Anhui Agricultural Sciences
2007,35
(10)
192-193+320
SYBR~ GreenI实时PCR鉴定检测转基因油菜RT73品系
作 者：
于莉;熊国华;刘志恒;曹际娟
单 位：
辽宁省出入境检验检疫局;沈阳农业大学
关键词：
SYBR~ GreenI实时PCR;转基因油菜;RT73品系;鉴定
摘 要：
利用特殊性引物,应用SYBR Green I实时PCR技术鉴定检测了商业化种植的转基因油菜RT73品系。结果表明,应用实时PCR技术,利用SYBR Green I染料能选择性结合双链DNA的特点,可检测到RT73品系特异性靶序列扩增所产生的荧光信号,且通过溶解曲线确定其熔点值为(80.2±0.5)℃,而对其他油菜品系则检测不到荧光信号。SYBR Green I实时PCR能通过溶解曲线有效地区分了特异性产物、非特异性产物以及引物二聚体,是基因鉴定检测的新方法。
译 名：
Identification for Genetically Modified Rape RT73 Line with SYBR~ Green I Real Time PCR
作 者：
YU Li et al (Shenyang Agricultural University,Shenyang,Liaoning 110161)
关键词：
SYBR Green I real-time PCR;Genetically modified rape;RT73 Line;Identification
摘 要：
The genetically modified rape RT73 line with commerce planting was identified by applying SYBR Green I real-time PCR assays and using specific primers.The results showed that the SYBR Green I could selectively combine the characteristic of double chain DNA and identify fluorescence signal produced by sequence amplification of specific target of RT73 line,and confirm the melting point of(80.2±0.5) ℃ by melting curve,while the fluorescence signal in other rape lines didn't be detected.The SYBR Green I real-time PCR could availably differentiate special or non-special products and primer dimers by melting curve,which is a new method for gene idenfification.
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