中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 鸭圆环病毒LAMP可视化检测方法的建立 中国动物检疫 2012,29 (3) 24-26
Position: Home > Articles > Development of Ioop-Mediated IsothermalAmplification Assay for Visual Detection of Duck Circovirus China Animal Health Inspection 2012,29 (3) 24-26

鸭圆环病毒LAMP可视化检测方法的建立

作  者：
赵光远;谢芝勋;谢丽基;庞耀珊;邓显文;刘加波;范晴
单  位：
广西大学动物科技学院;广西兽医研究所广西疫苗新技术重点实验室
关键词：
鸭圆环病毒;环介导等温扩增;可视化检测
摘  要：
根据GenBank中DuCV基因序列,在保守区设计了6条特异性引物,并对反应条件进行优化,建立了一种适用于鸭圆环病毒(DuCV)的环介导等温扩增快速检测方法(LAMP)。该方法对H9亚型禽流感、小鹅瘟、鸭瘟、鸭肝炎、鸭副黏病毒均无扩增反应;且扩增反应只需在常规水浴锅中进行,1小时内即可完成反应;对DuCV模版DNA的最小检测限为10fg,灵敏度是一步法PCR的1000倍。本研究建立的LAMP方法简便、快速、灵敏、特异,适合在基层进行DuCV的快速检测。
译  名：
Development of Ioop-Mediated IsothermalAmplification Assay for Visual Detection of Duck Circovirus
作  者：
Zhao Guangyuan 1,Xie Zhixun 2,Xie Liji 2,Pang Yaoshan 2,Deng Xianwen 2,Liu Jiabo 2,Fan Qing 2 (1.College of Veterinary Medicine,Guangxi University,Guangxi,Nanning 530004,China;2.Guangxi Veterinary Research Institute,Guangxi Key Laboratory of Anmial Vaccines and New Technology,Guangxi,Nanning 530001,China)
关键词：
duck circovirus;LAMP;visual detection
摘  要：
A loop-mediated isothermal amplification (LAMP) assay was developed for detection of duck circovirus(DuCV).According to the sequences of DuCV gene available in GenBank,six specific primers to gene were designed,and the reaction conditions were optimized.The results showed that the assay had no cross-reaction with H9 subtype AIV,gosling plague virus,duck plague virus,duck hepatitis virus,duck paramyxovirus and other grallatorial pathogens.The assay was performed in water bath within one hour and the amplification result was visualized by inspecting the white sediment and the green colour..The detection limit of LAMP assay was 10 fg,1 000-fold higher than that of the routine PCR.These results suggest that the LAMP assay is a simple and specific method for rapid detection of DuCV in field conditions.

相似文章

导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46