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农业科学与技术(英文)
2008,9
(1)
60-63,66
Position: Home > Articles > Development and characterization of monoclonal antibody specific to nuclear protein of avian influenza virus type A.
Agricultural Science & Technology
2008,9
(1)
60-63,66
Development and characterization of monoclonal antibody specific to nuclear protein of avian influenza virus type A.
作 者：
Li Na;Qin AiJian;Shao HongXia;Jin WenJie;Liu YueLong
单 位：
Yangzhou 225009; China.;College of Veterinary Medicine; Yangzhou University
关键词：
antigens;avian influenza;avian influenza A viruses;characterization;diagnosis;diagnostic techniques;ELISA;immunodiagnosis;immunofluorescence;immunological techniques;monoclonal antibodies;poultry;viral proteins;Influenza A virus
摘 要：
Five monoclonal antibodies (AIV-NP-2C3, AIV-NP-6A5, AIV-NP-3H9, AIV-NP-7B4 and AIV-NP-2H4) specifically against nuclear protein of avian influenzavirus (AIV) were developed by synthesizing the SP 2/0 and spleen cells of BALB mice immunized with H9 subtype AIV. The specificity of the monoclonal antibodies were analysed by IFA and ELISA. Results revealed that all monoclonal antibodies reacted with all AIV viral strains. It was observed that only the 60 ku protein antigen of AIV-H9 was recognized by the monoclonal antibodies using Western blot. Preliminary results showed that AIV can be detected by monoclonal antibodies in IFA and ELISA.
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