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Study on the purification process of total flavonoids from root of Rosa laevigata Michx. by macroporous adsorption resin.

作者：

Gongqing Wu;Yi YunHong;Zou MeiQing;Tian Su-ying;Zhen Zha

单位：

College of Life Sciences,Sun Yat-sen University,Guangzhou,Guangdong 510275,China;College of Life Sciences,Sun Yat-sen University,Guangzhou,Guangdong 510275,Chin

关键词：

total flavonoids;rosa laevigata michx.;root;macroporous adsorption resin;ab;purification proces

摘要：

[Objective] To obtain a suitable macroporous adsorption resin and optimize its purification process of total flavonoids from root of Rosa laevigata Michx..[Method] Effect of 4 resins like ADS-7,HPD300,AB-8 and D-101 on the adsorption and desorption of total flavonoids from root of Rosa laevigata Michx.were comparatively studied,and effects of concentration of loading solution and eluent concentration on desorption performance were investigated.[Result] AB-8 was the optimum macroporous adsorption resin for purifying total flavonoids from root of Rosa laevigata Michx..The optimum process to isolate and purify total flavonoids was that 20.0 ml of 0.200 mg/ml sample solution was adsorbed on the surface of AD-8 resin and eluted with 4 BV of 50% ethanol.Under the optimum conditions,the purification rate of total flavonoids from root of Rosa laevigata Michx.reached as high as 205.40%.[Conclusion] The high quality total flavonoids from root of Rosa laevigata Michx.were obtained in this work.

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Study on the purification process of total flavonoids from root of Rosa laevigata Michx. by macroporous adsorption resin.

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