当前位置: 首页 > 文章 > 烟草黑胫病菌对甲霜灵的抗药性风险 农药 2007,46 (10) 65-68
Position: Home > Articles > Resistance Risk of Phytophthora parasitica var. nicotianae to Metalaxyl Agrochemicals 2007,46 (10) 65-68

烟草黑胫病菌对甲霜灵的抗药性风险

作  者:
祝明亮;白江兰;李梅云;张克勤
单  位:
云南大学工业微生物发酵工程重点实验室;云南省烟草科学研究所
关键词:
烟草黑胫病菌;甲霜灵;抗药性风险;致病性;适合度
摘  要:
为了评估烟草黑胫病菌对甲霜灵的抗药性风险,以对甲霜灵敏感和高抗的烟草黑胫病菌240和191为出发菌株,进行了抗性突变频率测定、抗性诱变、抗性水平测定、遗传稳定性、生长速率、致病性测定和适合度等研究。结果表明,烟草黑胫病菌对甲霜灵敏感的240菌株在含10mg/L甲霜灵的CA平板上的抗性突变频率为7.0×10-5。分别以药剂诱变和紫外诱变获得了抗甲霜灵菌株240-YY和240-ZY。对甲霜灵敏感性不同的烟草黑胫病菌适合度测定结果表明,抗性菌株在潜伏期、侵染率、病情指数和病斑面积等方面均具有较高的适合度水平。研究结果显示,烟草黑胫病菌对甲霜灵具有较大的抗药性风险。
译  名:
Resistance Risk of Phytophthora parasitica var. nicotianae to Metalaxyl
作  者:
ZHU Ming-liang1,BAI Jiang-lan1,LI Mei-yun1,ZHANG Ke-qin2 (1.Yunnan Tobacco Science Research Institute,Yuxi 653100,Yunnan,China; 2.Key Lab of Industrial Microbiology Fermentation,Yunnan University,Kunming 650091,China)
关键词:
Phytophthora parasitica var. nicotianae; metalaxyl; resistance risk; pathogenicity; fitness
摘  要:
In order to evaluate resistance risk of Phytophthora parasitica var. nicotianae to metalaxyl,the metalaxyl-sensitive strain 240 and metalaxyl-resistant strain 191 were studied. The results showed that the mutation rate of metalaxyl-sensitive strain 240 was 7.0×10-5 on CA medium with metalaxyl concentration of 10 mg/L. The metalaxyl-resistant strains 240-YY and 240-ZY were obtained through mutagenesis of metalaxyl and ultraviolet radiation,respectively. The test results suggested that the metalaxyl-resistant strains of Phytophthora parasitica var. nicotianae had quite high level of fitness on their latent period,infection frequency,disease index and lesion area. The results indicated that there might be a high resistance risk for the Phytophthora parasitica var. nicotianae to metalaxyl.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊