中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响 茶叶科学 2003,23 (2) 119-123
Position: Home > Articles > Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice Journal of Tea Science 2003,23 (2) 119-123

儿茶素对铅诱导HepG2细胞氧化损伤及茶多酚对铅毒小鼠体内铅含量的影响

作  者：
朴宰日;王岳飞;杨贤强;陈留记;杨勇;赵保路;焦鸿利;周淡宜
单  位：
中国科学院生物物理研究所;浙江大学茶学系;浙江省医学科学院
关键词：
茶学;茶儿茶素;茶多酚;铅毒;脂质过氧化;排铅
摘  要：
观察茶儿茶素对铅诱导HepG2细胞氧化损伤和茶多酚对铅染毒小鼠体内铅含量的影响。采用MTT(四甲基偶氮唑盐)法检测细胞活力,TBA(硫代巴比妥酸)反应生成MDA(丙二醛)的量测量细胞脂质过氧化水平。以醋酸铅染毒小鼠,采用原子荧光光度法测定小鼠器官中的铅含量。结果发现儿茶素能改善铅诱导细胞的氧化损伤,使细胞活力升高,MDA下降;铅染毒后,小鼠血液、肝、肾、脑和股骨蓄积的铅含量显著增加,茶多酚对小鼠肝脏排铅有显著作用,但对其他器官的铅排泄无明显影响。
译  名：
Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice
作  者：
PIAO Zai-ri1,Wang Yue-fei1,YANG Xian-qiang1,CHEN Liu-ji1,YANG Yong1,ZHAO Bao-lu2,JIAO Hong-li2,ZHOU Dang-yi3 (1.Department of Tea Science, Zhejiang University, Hangzhou,310029, China; 2. Institute of Biophysics, Academia Sinica1, Beijing 100101, China; 3. Zhejiang Academy of Medical Sciences, Hangzhou 310013, China)
关键词：
Tea science; Tea catechins; Tea polyphenol; Lead exposure; Lipid preoxidation; Removal of lead
摘  要：
The effects of tea catechins on lead-induced lipid peroxidation damage in HepG2 cells and tea polyhenol on the content of lead in lead-exposed mice were investigated.HepG2 cell viability was determined using the MTT method,lipid peroxidation was assayed by detetmining the production rate of thibarbiturie acid reactive substance(TBARS)which was expressed as malondialdehyde(MDA)equivalents.The content of lead in lead-exposed mice was determined using hydride atomic fluorescence spectroscopic method. Results showed that tea catechins had protective effects on lead-exposed HepG2 cell viability and inhibitory effects on TBARS formation in HepG2 cells.All the indices of the mice in the exposed groups were significantly higher than those in the control.Tea polyphenol was able to accelerate the removal of lead from liver,but the same effects were not found in blood、kidney, brain and thigbones.

相似文章

导出引用
计量
文章访问数: 2
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46