Position: Home > Articles > Optimization of Enzymatic Preparation and Decolourization Technology of Yak Hemoglobin Bioactive Peptides
MEAT RESEARCH
2008
(4)
20-24
牦牛血红蛋白活性肽酶法制备工艺的优化及脱色技术研究
作 者：
孙骞;林巾侠;罗永康;刘昌蓉;白涛
单 位：
北京顺鑫农业股份有限公司;中国农业大学食品科学与营养工程学院
关键词：
牦牛血红蛋白;酶解;水解度;三氯乙酸可溶性氮;活性肽
摘 要：
为开发具有保健功能的新型高附加值生物制品,本实验采用胰蛋白酶、木瓜蛋白酶及AS1.398中性蛋白酶3种食品级内切蛋白酶对牦牛血红蛋白进行酶解,以水解度及三氯乙酸可溶性氮为评价指标,采用酶解-活性炭法对水解液进行脱色,筛选出AS1.398中性蛋白酶为最佳水解用酶。通过正交实验对其酶解工艺进行优化后,得出AS1.398中性蛋白酶最佳水解条件为:底物浓度5%,加酶量8000U/g,温度55℃,初始pH7.5,酶解时间5h。在此条件下水解产物为淡黄色,并具有较高的低分子量生物活性肽含量。
译 名：
Optimization of Enzymatic Preparation and Decolourization Technology of Yak Hemoglobin Bioactive Peptides
作 者：
Sun Qian1,Lin Jin-Xia1,Luo Yong-kang1,Liu Chang-rong2,Bai Tao2 (1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 10083,China;2. Beijing Shunxin Agricultural Company Limited, Beijing 101300,China )
关键词：
Yak hemoglobin;Enzymatic hydrolysis;Degree of hydrolysis;TCA-NSI;Bioactive peptides
摘 要：
In order to develop the biologicals of new-type high added value with health care function,this experiment adopts 3 food grade endo proteases named trypsin,papain and neutrase AS1.398 to hydroly-sis yak hemoglobin by degree of hydrolysis and TCA-NSI as the evaluation index,and this experiment adopts hydrolysis-activated carbon method to decolourization,and neutrase AS1.398 has been screened out as the greatest protease to hydrolysis yak hemoglobin.Orthogonal test is carried out to further optimize the best hydrolysis condition as follows:concentration of substrate 5%,neutrase AS1.398 added 8000 U/g,temperature 55℃,initial pH7.5, total hydrolysis time 5h.Under such conditions the hydrolysates is yellowish and the content of low molecular weight bioactive peptides of hydrolysates is relatively high.
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