Research on the Theory and Improvement of Mongolian Traditional Sauma Method

Chaganqiqige et al(College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018)

Mongolian tranditional sauma method;Horse race;Character candidate gene;Polymorphism

[Objective] The research aimed to combine Mongolian traditional sauma method with modern molecuar biotechnology so as to find a more scientific,simple and convenient sauma method.[Method] The correlation between polymorphism of single genes on COMT gene site of tested horses and their characters and characteristics was researched and the properties of character inheritance in horse were discussed in view of molecular biology.[Result] The electrophoresis detection showed that compared with Marker DL2000,the site of PCR products from the 2nd exon of COMT gene was below 250 bp.The results of PCR-SSCP analysis showed that the 2nd exon of COMT gene in horse genome DNA had single nucleotide polymorphism and there were 2 genotypes found in the 30 detected individuals,they were genotypes AA and AB.The sequencing results showed that there was a polymorphic site at the position 161 of the 2nd exon of COMT gene.The polymorphism of the 2nd exon of COMT gene had significant correlation with the competitiveness of horse and had no significant correlation with its other characters.The horse with genotype AB was more competitive than that with AA.[Conclusion] The research provided new technological support for the identification of horse.