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FOOD SCIENCE
2009,30
(5)
145-148
菜籽甾醇转化制备雄烯二酮高产菌株的诱变育种
作 者：
杨英;姜绍通;胡锦艳;赵俊平;梁铁艳
单 位：
合肥工业大学生物与食品工程学院
关键词：
菜籽甾醇;分枝杆菌;诱变育种;雄烯二酮
摘 要：
以分枝杆菌Mycobacterium sp.BD-696为出发菌株,采用紫外、硫酸二乙酯复合诱变处理,筛选得到两株雄烯二酮高产突变株:Mycobacterium sp.BD-696-3和Mycobacterium sp.BD-696-6,在底物甾醇添加量为6‰、摇瓶发酵168h时的雄烯二酮产量分别为2.31g/L和2.33g/L,得率分别为60.7%和61.4%,较出发菌株提高了12.8%和13.5%。连续传代实验结果表明,突变株Mycobacterium sp.BD-696-6的遗传性状稳定。
译 名：
Mutation Breeding of Strain Highly Producing Androst-4-ene-3,17-dione from Rapeseed Sterols
作 者：
YANG Ying1,2,JIANG Shao-tong1, HU Jin-yan1, ZHAO Jun-ping1,LIANG Tie-yan1 (1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China ; 2. Department of Environment Engineering, Anhui Institute of Architecture, Hefei 230022, China)
关键词：
rapeseed sterols;Mycobacterium sp;mutation breeding;androst-4 -ene-3,17-dione
摘 要：
The mutagenesis of Mycobacterium sp. BD-696 was induced by UV and DES, and two mutants Mycobacterium sp. BD-696-3 and Mycobacterium sp. BD-696-6 were obtained. When the concentration of substrate steroid is 6‰ and shaking flask fermentation is kept for 168 hours, the transformation yield of androst-4-ene-3,17-dione (AD) from rapeseed sterol by Mycobacterium sp.D-696-3 and Mycobacterium sp. BD-696-6 are 2.31 g/L and 2.33 g/L, respectively, and the corresponding transformation rates are 60.7 % and 61.4%, higher 12.8% and 13.5% than that by the original strain, respectively. Moreover, the continuous passage experiment showed that the mutant BD-696-6 has high genetic stability.
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