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当前位置: 首页 > 文章 > 石油降解菌群的构建及其对混合烃的降解特性 农业环境科学学报 2017,36 (03) 522-530
Position: Home > Articles > Construction of petroleum degrading bacteria consortium and its degradation properties of mixed hydrocarbons Journal of Agro-Environment Science 2017,36 (03) 522-530

石油降解菌群的构建及其对混合烃的降解特性

作  者：
范瑞娟;郭书海;李凤梅
单  位：
中国科学院沈阳应用生态研究所;北方民族大学生物科学与工程学院
关键词：
石油污染土壤;条件优化;生物修复;微生物群落
摘  要：
经富集、分离、纯化,并经选择性培养基的筛选和对总石油烃(TPH)降解能力的测试,从大庆油田石油污染土壤中获得6株分别具有环烷烃、直链烷烃和芳烃降解能力的菌株B_1、B_2、B_3、B_6、B_7和B_9。经16S r RNA基因序列比对,菌株B_1、B_2、B_6和B_7分别属于嗜氢菌属(Hydrogenophaga sp.)、苯基杆菌属(Phenylobacterium sp.)、鞘脂菌属(Sphingobium sp.)和芽胞杆菌属(Bacillus sp.),B_3和B_9属于节杆菌属(Arthrobacter sp.)。通过对接种比例、接种量和生长条件的优化,构建了微生物降解菌群,并采用该菌群进行混合烃污染土壤的修复。结果表明,该菌群对三类烃的去除均具有明显的促进作用。经过40 d的修复,环十二烷去除率达74.5%;100 d后,正十六烷和芘的去除率分别达56.9%和60.4%。前10 d修复过程中,各污染物降解速率最大,之后则逐渐降低,与土壤微生物多样性、数量以及活性的变化趋势相似。
译  名：
Construction of petroleum degrading bacteria consortium and its degradation properties of mixed hydrocarbons
作  者：
FAN Rui-juan;GUO Shu-hai;LI Feng-mei;College of Biological Science & Engineering, Beifang University of Nationalities;Institute of Applied Ecology,Chinese Academy of Sciences;
关键词：
petroleum contaminated soil;;optimal condition;;bioremediation;;microbial community
摘  要：
After enrichment, separation, purification, screening by selective medium, and testing by degradation ability of the total petroleum hydrocarbon(TPH), 6 strains(B_1, B_2, B_3, B_6, B_7 and B_9)with cyclanes, alkanes, and aromatics degradation ability were obtained. Based on the sequence analysis of 16 S r RNA gene, B_1, B_2, B_6 and B_7were identified as Hydrogenophaga sp., Phenylobacterium sp., Sphingobium sp.and Bacillus sp., respectively; B_3 and B_9were identified as Arthrobacter sp.. A microbial degradation flora was constructed through the optimization of the inoculum proportion, size, and growth conditions. The degradation flora was then used in the remediation of mixed hydrocarbon-contaminated soil, the results demonstrated an obvious effect of the flora in the degradation of three kinds of hydrocarbons. After 40 d of remediation, the degradation extent of cyclododecane can be reach to 74.5%; and after 100 d, the degradation extents of n-hexadecane and pyrene reached to 56.9% and 60.4%, respectively. There was a significant decrease of pollutants during the first 10 d, and the removal efficiency was reduced along with the experiment, showed a similar variation tendency with microbial diversity, quantity and activity in soil.

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