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当前位置: 首页 > 文章 > 利用蓝藻厌氧发酵液制备苏云金芽孢杆菌杀虫剂 农药 2014,53 (3) 27-31
Position: Home > Articles > Preparation of Bacillus thuringiensis Berliner Biopesticide by Using Biogas Slurry of Blue-green Algae Agrochemicals 2014,53 (3) 27-31

利用蓝藻厌氧发酵液制备苏云金芽孢杆菌杀虫剂

作  者：
谭超;张乃明
单  位：
云南农业大学植物保护学院;云南农业大学资源环境学院
关键词：
蓝藻;沼液;苏云金杆菌;杀虫剂
摘  要：
[目的]富营养化湖泊蓝藻暴发已成突出的环境问题,打捞蓝藻的处理和利用是目前的技术难题,探究蓝藻的农药化利用可行性。[方法]以新鲜蓝藻为基质自然厌氧发酵不同时间得到蓝藻沼液,并以4种培养基(LB、新鲜蓝藻、蓝藻发酵2个月沼液、蓝藻发酵6个月沼液)为原料,接种苏云金芽孢杆菌(B.thuringiensis GIM1.32),对比菌种在不同培养基中生长代谢特征、杀虫晶体蛋白产量,并以3龄小菜蛾为测试对象测定毒力效价。[结果]蓝藻自然厌氧发酵是一个产酸过程,自然状况下(20~25℃)直接打捞富集的蓝藻藻泥产气潜力为27.27 mL/g。随蓝藻自然厌氧发酵时间的增加,沼液中TS、VS、pH值、粗脂肪、蛋白质含量均呈现下降趋势,多糖含量呈现上升趋势,灰分变化不明显。当含固率3%、pH值为7、接种物种龄10 h、接种量2%、培养温度30℃时,不同阶段蓝藻沼液制备B.t.的晶体蛋白含量、LC50值、Tx均低于新鲜蓝藻培养基。[结论]新鲜蓝藻是B.t.GIM 1.32菌种的生长代谢良好的培养基,能够使其正常完成芽孢和伴孢晶体的合成,为蓝藻资源化利用提供了一条新的、高值处理利用途径,既将蓝藻变废为宝,又可有效降低苏云金芽孢杆菌生物农药生产成本。
译  名：
Preparation of Bacillus thuringiensis Berliner Biopesticide by Using Biogas Slurry of Blue-green Algae
作  者：
TAN Chaoa;ZHANG Nai-mingb;Yunnan Agricultural University,College of Plant Protection;Yunnan Agricultural University,College of Resources and Environments;
关键词：
blue-green algae;;biogas slurry;;Bacillus thuringiensis Berliner;;biopesticide
摘  要：
[Aims] Eutrophication outbreak of lake algae has become a prominent environmental problem. To salvage the processing and utilization of blue-green algae is the technical problems, to explore feasibility of pesticide use for bluegreen algae. [Methods] Fermented blue-green algae broth was obtained by natural anaerobic fermentation with fresh blue-green algae as medium. Thereafter, B. thuringiensis GIM 1.32 was inoculated into four kinds of medium(LB culture medium, fresh blue-green algae, 2-month fermented blue-green algae broth and 6-month fermented blue-green algae broth); metabolic characteristics of B. thuringiensis, production of insecticidal crystal protein and titer of pesticide toxicity were compared. [Results] Results showed that natural anaerobic fermentation of blue-green algae was an acid-producing process. The potential aerogenesis ability of blue-green algae was 27.27 mL/g underwent natural fermentation(20-25 °C). During natural anaerobic fermentation of blue-green algae fermentation, the TS, VS, pH value, crude fat and protein content of the fermentation broth showed a declining trend, whereas, the content of polysaccharide increased, meanwhile, ash content varied little. Under the condition of 3% solid content, pH 7, age of inoculation species 10 h, inoculation level 2%, and culture temperature 30 °C, B.t. crystal protein content, LC50, Tx of 2-month or 6-month fermented blue-green algae were much lower than fresh blue-green algae. [Conclusions] Blue-green algae is biological pesticides strains of B.t. GIM 1.32 a good medium. It can support the normal growth and metabolism, strain complete spore and spore crystal synthesis. This study provides the algae utilization a brand new, high value-added processing way, algae can be not only convert waste to useful resources, also can effectively reduce the B. thuringiensis biological pesticide production cost.

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