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PCR方法从进口饲料中检出转基因成分

作  者:
闻伟刚;贺水山;杜华宏;赵秀玲;孙立群
单  位:
宁波出入境检验检疫局
关键词:
进口饲料;泰农-40;效美素;PCR;转基因成分
摘  要:
本方法通过PCR扩增 ,从进口饲料泰农 - 40中得到 35S启动子和Nos终止子外源基因片段 ,同时 ,从进口饲料效美素中得到Nos终止子基因片段 ,经酶切验证 ,最后判定这两种饲料中均含有转基因成分。
译  名:
Transgenic ingredients identified in import feedstuffs by PCR
作  者:
Wen Weigang,He Shuishan,Du Huahong,Zhao Xiuling,Sun Liqun (Ningbo Entry-Exit Inspection and Quarantine Bureau,Ningbo 315012)
关键词:
import feedstuffs,Tainong-40,Xiaomeisu,PCR,transgenic ingredients
摘  要:
The 35S and Nos forgign genes were identified in Tainong-40 by PCR,and Nos gene was detected only in Xiaomeisu. Through the validate of endonuclease,this two sorts of import feedstuffs (Tainong-40 and Xiaomeisu) were determined to contain transgenic ingredients.

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