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当前位置: 首页 > 文章 > 防治蔬菜灰霉病解淀粉芽胞杆菌的分离鉴定及抑菌特性 农药 2013,52 (7) 490-493
Position: Home > Articles > Screening and Identification of Bacillus amyloliquefaciens against Vegetable Botrytis cinerea and Its Antimicrobial Characteristics Agrochemicals 2013,52 (7) 490-493

防治蔬菜灰霉病解淀粉芽胞杆菌的分离鉴定及抑菌特性

作  者：
吕钊;张丽萍;程辉彩;尹淑丽;张根伟
单  位：
河北省科学院生物研究所河北师范大学生命科学学院河北省主要农作物病害微生物控制工程技术研究中心;河北省科学院生物研究所
关键词：
蔬菜灰霉病;解淀粉芽胞杆菌;筛选;鉴定;抑菌特性
摘  要：
[目的]为研制防治蔬菜灰霉病的新型生物农药,进行高效生防菌的分离筛选及抑菌特性研究。[方法]通过形态学、生理生化和16S rDNA序列分析,对拮抗菌进行鉴定;采用平板扩散法测定发酵液的抑菌特性。[结果]分离筛选出1株对黄瓜灰霉病具有高效拮抗作用解淀粉芽胞杆菌(Bacillus amyloliquefaciens);该菌株在生长对数期产生抗菌物质,并在30-38 h达到最大值;产生的抗菌物质有很好的热、酸碱稳定性,紫外线照射不敏感,常温储存6个月活性保持在80%以上;抗菌谱测定表明其对黄瓜灰霉病菌(Botrytis cinerea)、番茄叶霉病菌(Fulvia fulva)、黄瓜炭疽病菌(Colletotrichum orbiculare)、番茄早疫病菌(Alternaria solani)等多种病原菌具有显著抑菌效果。[结论]该菌株所产抗菌物质性能稳定,抗菌谱宽,抑菌力强,具有开发成新型广谱生物农药的潜力。
译  名：
Screening and Identification of Bacillus amyloliquefaciens against Vegetable Botrytis cinerea and Its Antimicrobial Characteristics
作  者：
LYU Zhao1,2,ZHANG Li-ping2,3,CHENG Hui-cai2,3,YIN Shu-li2,3,ZHANG Gen-wei2,3,ZHAO Bao-hua1(1.College of Life Science,Hebei Normal University,Shijiazhuang 050016,China;2.Hebei Acad.of Sci.,Inst.of Biol,Shijiazhuang 050081,China;3.Microbial Ctrl.Engin.Technol.Res.Ctr.of Main Crops Dis.in Hebei Prov.,Shijiazhuang 050081,China)
关键词：
vegetable Botrytis cinerea;Bacillus amyloliquefaciens;screening;antibacterial characteristics;identification
摘  要：
[Aims] In order to develop a new biological pesticide against vegetable Botrytis cinerea,highly active antagonistic bacteria were screened and the antibacterial properties were investigated.[Methods] The strain was identified based on the morphological,physiological and biochemical characteristics and the 16S rDNA sequence,agar plate diffusion method was adopted to determinate the antibacterial properties of the fermentation broth.[Results] The strain isolated and screened from soil was identified as Bacillus amyloliquefaciens which had a highly activities antagonistic against B.cinerea.The antibacterial activity substance excreted by strain was produced in the logarithmic growth phase,and reached the highest between 30 to 38 hours.It got a thermal stability and pH stability,and was not sensitive to Ultraviolet.Stored for 6 months at room temperature the activity was maintained more than 80%.It had a highly active against B.cinerea,Alternaria solani,Fulvia fulva,Colletotrichum orbiculare and many other pathogenic bacteria.[Conclusions] The strain can be developed as a promising bio-control bacterium strain.

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