中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 绿茶多酚诱导肝癌细胞凋亡时Caspase-3蛋白活性与mRNA的变化 安徽农业科学 2008,36 (23) 104-106+194
Position: Home > Articles > Changes of Caspase-3 Activity and mRNA in EGCG-induced apoptotic Hep-G_2 cells Journal of Anhui Agricultural Sciences 2008,36 (23) 104-106+194

绿茶多酚诱导肝癌细胞凋亡时Caspase-3蛋白活性与mRNA的变化

作  者：
梁清清;石英;李卫国
单  位：
河南师范大学生命科学学院;新乡医学院免疫细胞实验室
关键词：
绿茶多酚;肝癌细胞Hep-G2;细胞凋亡;半胱天冬酶;蛋白活性
摘  要：
[目的]研究绿茶多酚(EGCG)诱导肝癌细胞Hep-G2凋亡过程中半胱天冬酶-3(Caspase-3)蛋白活性与mRNA的变化。[方法]100 mg/L的EGCG处理Hep-G2细胞48 h,DAPI染色观察细胞形态学,用MTT检测EGCG对Hep-G2细胞生长的影响,流式细胞技术Annexin V-FITC PI法检测不同浓度EGCG诱导细胞的凋亡率,分光光度法检测Caspase-3的活性,用半定量RT-PCR法检测Caspase-3的mRNA变化。[结果]100 mg/L的EGCG作用Hep-G2细胞48 h后,荧光显微镜观察到细胞出现核碎裂和凋亡小体;MTT法检测到EGCG对肝癌细胞Hep-G2的生长均有显著抑制作用,呈浓度依赖性;Annexin V-FITC PI法检测到不同浓度的EGCG作用与肝癌细胞Hep-G2后,凋亡率较对照组明显升高,并随诱导浓度的增加而增加;不同浓度的EGCG处理组和不同时间EGCG处理组的Caspase-3活性和mRNA表达量均比对照组明显升高。[结论]Caspase-3蛋白活性与mRNA上调是EGCG诱导肝癌细胞凋亡的的重要原因和机制。
译  名：
Changes of Caspase-3 Activity and mRNA in EGCG-induced apoptotic Hep-G_2 cells
作  者：
LIANG Qing-qing et al(College of Life Science,Henan Normal University,Xinxiang,Henan 453007)
关键词：
EGCG;Hepatoma cells Hep-G2;Apoptosis;Caspase-3;Protein activity
摘  要：
[Objective] This paper was conducted to analyze the activity of Caspase-3 and mRNA translation in the EGCG-induced apoptotic.[Method] Hep-G2 cells were incubated with EGCG 100 mg/L for 48 h.The cellular morphological analysis was conducted by DAPI pigmentation.The infection of EGCG on the growth of Hep-G2 was tested by MTT.The cellular apoptotic rate was analyzed at different dose by a flow cytometry(Annexin V-FITC PI).Activity of caspase-3 was measured using a colorimetric method.The mRNA level was semi-quantified by a reverse transcriptase-polymerase chain reaction technique.[Result] After treated with EGCG 100 mg/L for 48 h,the Hep-G2 cells declined its shape with regard to nuclear fragmentation and apoptotic body by fluorescence microscope.EGCG inhibited proliferation of Hep-G2 cells in dose by MTT assay.Compared with the control,the apoptosis ratio of Hep-G2 cells incubated with EGCG increased by dose.The activity of caspase-3 and the mRNA level of the EGCG-treated Hep-G2 cells at different times and different doses were significantly higher than the those of control(P<0.01).[Conclusion] EGCG led to the apoptosis of Hep-G2 cells by up-regulating activity of caspase-3 and mRNA level.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46