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当前位置: 首页 > 文章 > 吉氏巴贝斯虫多个GPI锚定蛋白基因的筛选与生物信息学分析 中国动物传染病学报 2019 (5) 57-64
Position: Home > Articles > SCREENING AND ANALYSIS OF BABESIA GIBSONI GLYCOSYLPHOSPHATIDYLIONSITLO(GPI)-ANCHORED GENES BY BIOINFORMATICS Chinese Journal of Animal Infectious Diseases 2019 (5) 57-64

吉氏巴贝斯虫多个GPI锚定蛋白基因的筛选与生物信息学分析

作  者：
战雪燕;聂政;赵阳楠;罗晓莹;李慕晓;安晓梦;敖阳思琦;崔杰;王森;贺兰;赵俊龙
单  位：
华中农业大学动物医学院
关键词：
吉氏巴贝斯虫;GPI锚定蛋白;生物信息学;免疫原性
摘  要：
吉氏巴贝斯虫是一类红细胞内专性寄生的血液原虫,犬一旦感染终生带虫,治疗后时有复发,因此需要简便快捷的临床诊断方法及时确诊治疗。GPI锚定蛋白是虫体表面重要的抗原分子,是很好的潜在诊断标识,在虫体粘附、识别以及入侵红细胞等过程中具有非常重要的作用。为了筛选吉氏巴贝斯虫GPI锚定蛋白基因,通过搜索NCBI、Uniprot以及PiroplasmaDB数据库中吉氏巴贝斯虫表面抗原,与武汉株吉氏巴贝斯虫数据库作比对,筛选出可能的吉氏巴贝斯虫武汉株的GPI锚定蛋白基因,并通过bigPI、PredGPI、GPI-SOM网站预测其GPI锚定位点。运用生物信息学分析软件预测其氨基酸信号肽、跨膜区、疏水区以及结构域和抗原表位等,分析其生物信息特性。通过分析确定了5个吉氏巴贝斯虫武汉株的GPI候选抗原,分别命名为BgP50-WH、BgP47-WH、BgP45-WH、BgP32-WH、BgP12-WH,其GPI锚定位点均位于C端,长度约为21～22个氨基酸,均有信号肽并含有4个抗原表位。分析结果表明,这5个GPI抗原均有望成为吉氏巴贝斯病的诊断标识,其中BgP47-WH和BgP50-WH与抗体亲和力最强,最具有成为诊断标识的潜力。
译  名：
SCREENING AND ANALYSIS OF BABESIA GIBSONI GLYCOSYLPHOSPHATIDYLIONSITLO(GPI)-ANCHORED GENES BY BIOINFORMATICS
作  者：
ZHAN Xue-yan;NIE Zheng;ZHAO Yang-nan;LUO Xiao-ying;LI Mu-xiao;AN Xiao-meng;AO Yang-si-qi;CUI Jie;WANG Sen;HE Lan;ZHAO Jun-long;College of Veterinary Medicine, Huazhong Agricultural University;
关键词：
Babesia gibsoni;;GPI-anchored protein;;bioinformatics;;immunogenicity
摘  要：
Babesia gibsoni is a haemoparasite infected RBC, causing babesiosis in dogs. The infected dogs carry the parasites for the rest of life and occasionally become clinically sick. Therefore, simple and fast diagnostic method is required for clinical diagnosis. Glycosylphosphatidylinositol(GPI)-anchored proteins important antigens on the surface of the parasites, playing significant roles in parasitic adhesion, recognition and invasion to host RBCs. Thus, GPI-anchored proteins could be potential diagnostic markers for detecting B. gibsoni. In order to identify B. gibsoni GPI-anchored coding genes, B. gibsoni genome was searched in NCBI, Uniprot and PiroplasmaDB. Then, GPI-anchor sequences were predicted by bigPI, PredGPI, GPI-SOM websites and BLAST and alignments were performed based on the obtained sequences. The signal peptides, transmembrane domains, hydrophobicity and protein domains and the antigen epitopes were also predicted by bioinformatics analysis software. As a result, 5 B. gibsoni GPI-anchored protein coding genes BgP50-WH, BgP47-WH, BgP45-WH, BgP32-WH and BgP12-WH were obtained. Further analysis confirmed that all 5 genes had a 21-22 aa GPI in C-terminal, and contained a signal peptide and multiple epitopes. The protein encoding genes, especially BgP47-WH and BgP50-WH containing multiple epitopes might be used as candidates for diagnostic antigens.

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