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Position: Home > Articles > Response Surface Methodology for Optimization of Maize Resistant Starch Preparation with Pullulanase FOOD SCIENCE 2011 (22) 11-15

响应面法优化玉米抗性淀粉制备工艺

作  者:
张焕新;于博;金征宇
单  位:
江苏畜牧兽医职业技术学院食品科技学院;湖北文理学院化学工程与食品科学学院;江南大学食品学院
关键词:
抗性淀粉;普鲁兰酶;玉米淀粉;响应面
摘  要:
研究普鲁兰酶法制备玉米抗性淀粉的工艺。在单因素试验基础上,采用响应曲面法研究pH值、反应温度、反应时间和加酶量对抗性淀粉得率的影响,优化玉米抗性淀粉制备工艺,建立各因素与抗性淀粉得率关系的数学回归模型。确定最佳的制备工艺条件为普鲁兰酶加酶量12.8ASPU/g、反应时间32h、反应温度46.2℃、p H 5.0。在该制备条件下,抗性淀粉得率为4 6.2%。
译  名:
Response Surface Methodology for Optimization of Maize Resistant Starch Preparation with Pullulanase
作  者:
ZHANG Huan-xin1,2,YU Bo1,JIN Zheng-yu1,(1.State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University, Wuxi 214122,China;2.School of Food Science and Technology,Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300,China)
关键词:
resistant starch;pullulanase;maize starch;response surface methodology(RSM)
摘  要:
In this study,resistant starch was prepared from maize starch with pullulanase.Based on one-factor-at-a-time experiments,the main parameters of pullulanase hydrolysis were investigated by response surface methodology(RSM),including reaction temperature,pH value,reaction time,and pullulanase amount,and a mathematical model describing resistant starch yield was developed.The optimum preparation conditions were achieved as follows: pullulanase amount 12.8 ASPU/g,reaction temperature 46.2 ℃,reaction time 32 h,pH 5.0.Under these conditions,the yield of the maize resistant starch is 46.2%.

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