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Position: Home > Articles > Determination of Glycyrrhizic Acid Content in Qingfei Granule by HPLC Modern Agricultural Science and Technology 2020 (7) 222-223+225

高效液相色谱法测定清肺颗粒中甘草酸的含量

作  者:
许世富
单  位:
安徽省兽药饲料监察所
关键词:
清肺颗粒;甘草酸;HPLC
摘  要:
利用高效液相色谱法测定清肺颗粒中甘草酸的含量。采用C18柱(5μm,4.6 mm×250 mm),在室温下,以甲醇-0.2 mol/L醋酸铵溶液-冰醋酸(66∶33∶1,V/V/V)为流动相,250 nm为检测波长,流速1.0 mL/min。结果表明,甘草酸在4.90~195.90μg/mL范围内,线性关系良好(r=0.999 9),平均回收率为91.97%,RSD为3.41%。本方法简单快捷、精密度好,可用于控制清肺颗粒中甘草酸的含量。
译  名:
Determination of Glycyrrhizic Acid Content in Qingfei Granule by HPLC
作  者:
XU Shi-fu;Anhui Provincial Control Institute of Veterinary Pharmaceuticals and Feed;
关键词:
qingfei granule;;glycyrrhizic acid;;HPLC
摘  要:
The content of glycyrrhizic acid in qingfei granule was determined by HPLC.Using C18 column(5 μm,4.6 mm×250 mm)at room temperature,methanol-0.2 mol/L ammonium acetate solution-glacial acetic acid(66∶33∶1,V/V/V)was used as mobile phase,the detection wavelength was 250 nm,the flow rate was 1.0 mL/min.The results showed that the glycyrrhizic acid had a good linear relationship(r=0.999 9) in the range of 4.90~195.90 μg/mL,the average recovery was 91.97% and RSD was 3.41%.The method is simple,rapid and accurate,and can be used to control the content of glycyrrhizic acid in qingfei granules.

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