Position: Home > Articles > Effects of Oxygen Supply on the Growth of Saccharomyces cerevisiae and S-adenosyl-L-methionine Formation
Hubei Agricultural Sciences
2008,47
(9)
1081-1084
氧供给对啤酒酵母生长和S-腺苷甲硫氨酸合成的影响
作 者：
汪文俊
单 位：
生物技术国家民委重点实验室/中南民族大学生命科学学院
关键词：
体积氧传递系数;溶解氧分压;S-腺苷甲硫氨酸;啤酒酵母
摘 要：
在5L发酵罐中不同体积氧传递系数(KLa)和溶解氧分压对高产腺苷甲硫氨酸的啤酒酵母(S.cere-visiae)生长和S-腺苷甲硫氨酸(SAM)合成的影响进行了研究。结果表明,不同KLa和溶解氧分压对S.cerevisiae的生长和SAM合成有着显著的影响,较高的KLa和溶解氧分压更有利于生长和SAM合成。在初始KLa为148.5 h-1时,S.cerevisiae的生物量、SAM产量和含量最高,分别为19.37 g·L-1、1.99 g·L-1和103 mg.g-1 DCW。在溶解氧分压为较高的50%时,S.cerevisiae的生物量、SAM产量较高,最大值分别为18.57 g·L-1和1.91 g·L-1。
译 名：
Effects of Oxygen Supply on the Growth of Saccharomyces cerevisiae and S-adenosyl-L-methionine Formation
作 者：
WANG Wen-jun(Key Lab for Biotechnology of the State Ethnic Affairs Commission/College of Life Science,South-Central University for Nationalities,Wuhan 430074,China)
关键词：
KLa;DOT;SAM;Saccharomyces cerevisiae
摘 要：
The effects of oxygen supply on growth of Saccharomyces cerevisiae and S-adenosyl-L-methionine(SAM) formation were studied in 5 L fermentor.Initial volumetric oxygen transfer coefficient(KLa) values within the range of 21.5～148.5 h-1 had significant effects on the cell growth and SAM formation,the increase of initial KLa led to more SAM accumulation and faster growth of S.cerevisiae.At initial KLa of 148.5 h-1,the maximal cell concentration,SAM production and content were 19.37 g·L-1,1.99 g·L-1 and 103 mg·g-1 DCW,repectively.Dissolved oxygen tension(DOT) also remarkably affected the fermentation process.The cell growth of S.cerevisiae was significantly inhibited when DOT was controlled at 20% of air saturation,which was due to the oxygen limitation in broth.The SAM yield and content at 50% of DOT were higher than those at 20% of DOT,the optimal biomass and SAM yields were 18.57 g·L-1 and 1.91 g·L-1.
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