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Position: Home > Articles > An Improved Method for Determination of Lycium barbarum Polysaccharides FOOD SCIENCE 2013,34 (12) 292-295

枸杞多糖组成及含量测定方法的改进

作  者:
刘源才;孙细珍;许银;李先芝;严玲
单  位:
劲牌有限公司
关键词:
枸杞多糖;真伪;组成;含量;检测方法
摘  要:
分析当前通行的枸杞多糖含量测定方法,加以优化与改进。将枸杞多糖进行水解衍生化处理后用气相色谱法和高效液相色谱法测定单糖组成,根据组成结果判别枸杞多糖的真伪;在此基础上,根据各单糖组成物质的量比配制单糖混合溶液,用苯酚-硫酸比色法绘制标准曲线,建立枸杞多糖含量测定方法,测定枸杞多糖含量为30%,而仅以葡萄糖做标准溶液,用硫酸-苯酚法绘制标准曲线,枸杞多糖含量仅为14%。
译  名:
An Improved Method for Determination of Lycium barbarum Polysaccharides
作  者:
LIU Yuan-cai,SUN Xi-zhen*,XU Yin,LI Xian-zhi,YAN Ling(Jing Brand Co.Ltd.,Daye 435100,China)
关键词:
Lycium barbarum;polysaccharides;authenticity;composition;content;determination method
摘  要:
An improved phenol-sulfuric acid method for the determination of Lycium barbarum polysaccharides was proposed by using mixed monosaccharide standards instead of glucose alone for prepration of a standard curve.The monosacccharide composition of samples was determined by HPLC after hydrolysis and subsequent derivatization for disrimination between real and fake Lycium barbarum polysaccharides.The polysaccharide content of LBP-1,a polysaccharide extract of Lycium barbarum prepared in our laboratory,was determined by the improved method to be 30% compared to only 14% with the traditional method.

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