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齿瓣石斛组织培养技术研究

作  者:
高燕;李守岭;白燕冰;李泽生;耿秀英
单  位:
云南省德宏热带农业科学研究所
关键词:
齿瓣石斛;种胚;组织培养
摘  要:
以齿瓣石斛蒴果为外植体进行胚培养的试验表明,初始萌发培养基为1/2MS;原球茎萌发培养基为3/4MS+10%马铃薯汁+5%香蕉汁+6-BA0.2mg·L-1+NAA0.05mg·L-1+0.05%AC;继代增殖培养基为MS+10%马铃薯汁+10%香蕉汁+6-BA0.5mg.L-1+NAA0.2mg.L-1+0.1%AC;壮苗生根培养基为1/2MS+10%香蕉汁+5%马铃薯汁+6-BA0.2mg·L-1+NAA1.0mg·L-1+0.1%AC,生根诱导率达100%,成活率达90%以上。
译  名:
Study on Dendrobium devonianum in Vitro
作  者:
GAO Yan,LI Shou-ling,BAI Yan-bing,LI Ze-sheng,GENG Xiu-ying Dehong Institute of Tropical Agriculture,Ruili 678600,China
关键词:
Dendrobium devonianum;embryo breeding;tissue culture
摘  要:
Experiment of Dendrobium devonianum was carried out in Dehong.Results indicated that the optimum medium were 3/4MS+6-BA0.2 mg·L-1+NAA0.05 mg·L-1+10% banana juice+5% potato juice,MS+6-BA0.5 mg.L-+NAA0.2 mg·L-+10% banana juice+10% potato juice and 1/2MS +6-BA0.2 mg·L-+NAA1.0 mg·L-+10% banana juice +5% potato juice for seedling,propagation culture and rooting respectively.Root inducing rate reaches 100% and survival rate is up to 90% after transplanting.

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