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当前位置: 首页 > 文章 > Pb在类芦组织和亚细胞中的分布规律和毒害效应 农业环境科学学报 2016,35 (11) 2077-2085
Position: Home > Articles > The distribution and toxic effects of Pb at the levels of the tissue and sub-cellular in Neyraudia reynaudiana Journal of Agro-Environment Science 2016,35 (11) 2077-2085

Pb在类芦组织和亚细胞中的分布规律和毒害效应

作  者：
黄玫英;罗洁文;黄彩凤;周垂帆
单  位：
福建农林大学林学院
关键词：
Pb;类芦;组织结构;亚细胞;SEM;TEM-EDS
摘  要：
通过研究类芦体内Pb在组织和亚细胞水平的毒害效应和分布规律,分析类芦对Pb的耐性机制,为了解类芦对重金属的富集能力、耐性机制及逆境生理提供理论依据。采用营养液培养的方法,以Pb为目标污染物,胁迫后测定类芦体内(分为根和叶)Pb在各亚细胞组分的含量,并分别通过扫描电子显微镜(SEM)和能谱透射电子显微镜(TEM-EDS)观察Pb在组织和亚细胞的分布以及对其损伤情况。结果显示:从组织水平来看,Pb胁迫下组织结构由排列整齐变为不规则,局部出现较大破损而形成一些碎片,并出现晶体堵塞导管的现象;从亚细胞水平来看,类芦根叶细胞均损伤,出现细胞壁模糊、线粒体减少、叶绿体肿胀等现象,在根部Pb主要分布于细胞壁(12.28 mg·kg~(-1))和以液泡为主的可溶组分(38.82 mg·kg~(-1)),而叶片中以可溶组分占比例最大(32.56%),且EDS元素分析也显示细胞内含有大量Pb。这些结果表明,类芦对Pb有一定的吸收富集能力,并可通过改变Pb在其组织和亚细胞水平的分布来降低毒害作用,因此类芦对重金属Pb有较强的耐性。
译  名：
The distribution and toxic effects of Pb at the levels of the tissue and sub-cellular in Neyraudia reynaudiana
作  者：
HUANG Mei-ying;LUO Jie-wen;HUANG Cai-feng;ZHOU Chui-fan;College of Forestry, Fujian Agriculture and Forestry University;Co-innovation Center for Soil and Water Conservation in Red Soil Region of the Cross-straits;
关键词：
Pb;;Neyraudia reynaudiana;;tissue;;sub-cellular;;scanning electron microscope(SEM);;transmission electron microscope with energy dispersive x-ray spectroscopy(TEM-EDS)
摘  要：
In order to examine the phytoremediation potential of Neyraudia reynaudiana,the study explored the toxic effects and distribution of lead(Pb)at the levels of the tissue and sub-cellular in this species and analyzed the supplementing the growth medium with Pb as a tar-get pollutant. After cultivation was concluded,the uptake of sub-cellular Pb was determined,and the distribution of Pb as well as the Pb-induced damage was respectively assessed through scanning electron microscope(SEM) and transmission electron microscope with energy dispersive X-ray spectroscopy(TEM-EDS). Pb caused the tissues to become irregular and fragmented by injuries;additionally,a portion of the catheters were filled with crystals.At the sub-cellular level,many phenomena demonstrated that leaf and root cells were harmed;for example,cell walls became vague,mitochondria were less numerous,and chloroplasts were swollen. Pb was mainly distributed in the cell wall(12.28 mg·kg~(-1))and the vacuole(38.82 mg·kg~(-1))of root cells,and it accounted for 32.56% of the total biomass in the leaves.In addition,the EDS analysis found a significant amount of Pb in cells.These results suggest that N.reynaudiana has a strong ability to both absorb and tolerate Pb. Moreover,the species may alter the distribution of Pb at both the tissue and the sub-cellular levels to reduce toxicity.Therefore,N.reynaudiana is well suited for phytoremediation in Southern China and elsewhere.

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