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Position: Home > Articles > Optimization of Tissue Culture Regeneration System of Eucommia ulmoides Journal of Northwest Forestry University 2008,23 (4) 97-100

杜仲组织培养再生体系的优化

作  者:
李俊红;张焕玲;李周岐
单  位:
西北农林科技大学林学院
关键词:
杜仲;组织培养;再生体系;优化
摘  要:
以幼嫩叶片为外植体,对杜仲再生体系的优化进行了研究。结果表明:叶片诱导愈伤组织的最适宜培养基为MS+NAA1.5 mg/L+BA2.0 mg/L,诱导率为95%、愈伤组织增殖最适宜培养基为MS+NAA1.0 mg/L+BA2.0 mg/L,良好率为98.5%、诱导不定芽的最适宜培养基为MS+NAA0.5 mg/L+BA2.0 mg/L,分化率为40%、最适宜生根培养基1/2MS+IBA1.5 mg/L,生根率为92.5%。
译  名:
Optimization of Tissue Culture Regeneration System of Eucommia ulmoides
作  者:
LI Jun-hong,ZHANG Huan-ling,LI Zhou-qi(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
Eucommia ulmoides;tissue culture;regeneration syetem;optimization
摘  要:
Optimization of regeneration system of Eucommia ulmoides was studied by using tender leaves as explants.The results showed that MS+NAA1.5 mg/L +BA2.0 mg/L had the best effects on induceing callus with a inducing rate of 95%,the best medium of callus growth was MS+NAA1.0 mg/L +BA2.0 mg/L with a conserve rate of 98.5%,the suitable medium for bud formation was MS medium added NAA0.5 mg/L,BA2.0 mg/L,with a differentiation rate of 40%,the optimum media for rooting is 1/2MS+IBA1.5 mg/L with 92.5% rooting ratio.

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