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Position: Home > Articles > Application of profitable microbe in healthy cultivation of Hippocampus kuda Journal of Fishery Sciences of China 2003 (1) 46-50

有益微生物在大海马健康养殖中的应用研究

作  者：
吕军仪;孙燕燕;李秉记;吴金英;黄克;陈琳
单  位：
广东中大亿达洲生物科技股份有限公司;中山大学生命科学学院广东省水生经济动物良种繁育重点实验室
关键词：
有益微生物;大海马;健康养殖
摘  要：
实验用复合微生物由芽孢杆菌(Bacillusspp.)、红螺菌(Rhodospirillumspp.)、硝化细菌(Nitrobacterspp.)和硫化细菌(Thiobacillusspp.)组成,菌量比例分别为61.5%、27.3%、5.8%和5.4%。向大海马养殖池每10d使用1次,用量5mL/m3,菌液密度(2.0～2.5)×109/mL。结果表明,应用复合微生物的试验组溶解氧比对照组(不加菌)提高了32.7%,氨氮、亚硝酸盐和硫化物含量则分别降低了76.9%、97.1%和93.3%。对照池的浮游藻类优势种为蓝藻、甲藻和隐藻,分别占浮游植物总量的35.3%、22.3%和19.6%;试验池的浮游藻类优势种为绿藻和硅藻,分别占69.0%和26.8%,其藻类多样性指数比对照组增大了1.2倍。试验池的异养菌增长率为55.3%,而对照池的异养菌增长率达274.6%。试验期间,水温23～26℃,试验组的芽孢杆菌的数量从0.15×103CFU/mL急剧增至55.63×103CFU/mL,但对照组的仍保持在(0.13～0.38)×103CFU/mL的水平。而试验组弧菌数量比对照池的降低了2个数量级。
译  名：
Application of profitable microbe in healthy cultivation of Hippocampus kuda
作  者：
LU Junyi1, SUN Yanyan1, LI Bingji2, WU Jinying1, HUANG Ke2, CHEN Lin2 atic Economic Animals, School of Life Science,Zhongshan University, Guangzhou 510275, China;2. Guangdong Zhongda Richvast Biological Science & Technology Corporation, Lufeng 516500, China)
关键词：
profitable microbe; Hippocampus kuda; healthy cultivation
摘  要：
Bacillus spp., Rhodospirillum spp., Nitrobacter spp. and Thiobacillus spp. were employed as mixed profitable microbe and the composition was 61.5%, 27,3%, 5.8% and 5.4%, respectively.The water temperature was kept at 23-26 ℃. The mixed microbe was applied to the culture water of Hippocampus kuda each time in ten days intervals and the density was (2.02.5)×109 /mL and the trial period was one month. The results showed that the DO in the experiment ponds increased by 32.7% compared with the control (without adding the mixed microbe), and the concentrations of NH4+N, NO-2 N and sulfide decreased by 76.9%, 97.1% and 93.3% , respectively. Meanwhile, the predominant species of algae in the control pond were Cyanophyta (35.3%), Pyrrophyta (22.3%) and Cryptophyta (19.6%) while in the experiment ponds were Cholrophyta (69.0%) and Bacillariophyta (26.8%) and the diversity index of algae increased to 1.2 times of the control. The growth rate of heterotrophic microbe in the experiment ponds was only 55.3% but in the control that was 274.6%. With the use of profitable microbe the number of Bacillus spp. increased rapidly from 0.15×103 CFU/mL to 55.63×103 CFU/mL while that in the control still remained at (0.13-0.38)×103 CFU/mL. The total number of Vibrio spp. in the experiment ponds reduced by 102 times. The growth rates of body length and body weight and the survival rate of H. kuda in the experiment ponds increased by 64.9%, 72.3% and 78.8%, respectively, compared with the control.

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