Effects of Pseudomonas sp. HYS on the Germination of Rapeseed

JIANG Liu;LIU Bin;WANG Rui;XIE Zhi-xiong;No.1 Middle School Affiliated,Central China Normal University;College of Life Sciences,Wuhan University;

Pseudomonas sp.;;HYS strain;;rapeseed(Brassica napus L.);;germination

In order to study the effect of Pseudomonas sp. HYS on the germination of rapeseed,HYS bacteria were cultured in LB medium,and the rape seeds were soaked with water(control),LB,and HYS culture liquid,respectively. The results showed that soaking seeds with HYS strain did not affect the split number and the number of shoots at last,but inhibited the splitting and shooting progress of rapeseed. HYS strain inhibited the splitting progress of rapeseed,the rape germination and the growth of rape shoot when the rapeseeds were contaminated with HYS strain during germination. LB inhibited the growth of rape root. HYS strain inhibited the rape germination when the rapeseeds were contaminated with HYS strain during germination of 1~2 d,earlier contamination enhanced the inhibition effect;continuous contamination reinforced the inhibition effect.However,the inhibition effect of HYS strain occurred only during 1~3 days of rape germination. Rape seeds were cultivated by diluted LB and supernatant of HYS bacteria liquid that was treated with centrifugal filter,the results showed that 100 times or 1 000 times diluents of HYS supernatant did not affect the split number of rapeseed,but accelerated rape germination and was beneficial to the growth of rape shoot. The results indicated that the Pseudomonas sp. HYS should not be used during the germination and early stage of growth in rape,however,diluted supernatant of HYS culture liquid could be used to accelerate the rape germination and promote the growth of rape shoot.