中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 氯苯类胁迫对蚕豆幼苗超氧化物歧化物活性的影响 农业环境科学学报 2004,23 (03) 432-436
Position: Home > Articles > Effect of Stress from Chlorobenzenes on SOD Activity of Broadbean Seedlings Journal of Agro-Environment Science 2004,23 (03) 432-436

氯苯类胁迫对蚕豆幼苗超氧化物歧化物活性的影响

作  者：
刘宛;李培军;周启星;孙铁珩;台培东;许华夏
单  位：
中国科学院沈阳应用生态研究所陆地生态过程重点实验室
关键词：
土壤;氯苯类污染;蚕豆;SOD活性;生物标记物
摘  要：
采用室内培养方法,研究了土壤中不同浓度氯苯(CB)、1,2,4-三氯苯(TCB)和六氯苯(HCB)胁迫对蚕豆幼苗生长、超氧化物歧化酶(SOD,EC1.15.1.1)活性及丙二醛(MDA)含量变化的影响。结果表明,50～300μg·g-1TCB处理5d后蚕豆幼苗生长受到抑制(P<0.05和P<0.01)。50～300μg·g-1TCB胁迫1～5d对蚕豆幼苗SOD活性的影响表现出明显的剂量-效应关系。TCB处理5d后的幼苗恢复培养3d,50μg·g-1TCB处理组的SOD活性得到恢复,而100和300μg·g-1TCB处理组的SOD活性明显高于对照(P<0.05和P<0.01)。试验亦表明,100和300μg·g-1TCB处理3d和5d,幼苗MDA含量均比对照显著增加(P<0.05和P<0.01),并且与TCB浓度之间存在明显的正相关关系。上述结果表明,蚕豆幼苗SOD活性及MDA含量可作为短期土壤TCB污染的生物标记物,而50～1000μg·g-1CB或HCB处理5d对蚕豆幼苗没有产生明显的毒性效应。
译  名：
Effect of Stress from Chlorobenzenes on SOD Activity of Broadbean Seedlings
作  者：
LIU Wan, LI Pei jun, ZHOU Qi xing, SUN Tie heng, TAI Pei dong, XU Hua xia(Key Laboratory of Terrestrial Ecological Processes, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China)
关键词：
soil; chloro- benzenes contamination; broadbean; SOD activity; Biomarkers
摘  要：
Root growth, changes in superoxide dismutase (SOD, EC 1.15.1.1) activity, malonyldialdehyde (MDA) level of broadbean seedlings were evaluated at different concentrations of chloro- benzene (CB), 1,2,4- trichlorobenzene (TCB) and hexachloro- benzene (HCB) in a soil. The results showed that root growth of seedlings was interrupted after 5 d at concentrations of 50 ～ 300 μ g· g- 1 with TCB treatment. The effects of TCB stress on SOD activity in seedlings displayed significant dose- effect relationship during the period of 1～ 5 d at concentrations of 50～ 300 μ g· g- 1. When broadbean seedlings were released from 5 d of TCB stress to a clear tap- water for 3 d, SOD activity at 50 μ g· g- 1 TCB recovered to the control level (P >0.05) while a significant increase in SOD activity was observed at 100 and 300 μ g· g- 1 TCB, compared to control (P< 0.05 and P< 0.01, respectively). The experiments also revealed that a significant increase of MDA level in seedlings occurred after 3 d and 5 d of 100 and 300 μ g· g- 1 TCB treatment (P< 0.05 and P< 0.01), and there was a positive correlation between TCB concentrations and MDA levels. All the above results indicated that SOD activity and MDA level of broadbean seedlings may be proposed as biomarkers for short- term TCB contamination in soil. Compared to TCB, the toxicity of 50 ～ 1 000 μ g· g- 1 CB or HCB in soil to broadbean seedlings was not found after a 5 d exposure.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46