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当前位置: 首页 > 文章 > 壳寡糖诱导对烟草体内TMV-CP基因表达的抑制作用 植物病理学报 2007,37 (6) 637-641
Position: Home > Articles > Inhibitory effect to TMV-CP gene expression in tobacco induced by chito-oligosaccharides Acta Phytopathologica Sinica 2007,37 (6) 637-641

壳寡糖诱导对烟草体内TMV-CP基因表达的抑制作用

作  者：
商文静;吴云锋;商鸿生;赵小明;杜昱光
单  位：
西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室;中国科学院大连化学物理研究所
关键词：
壳寡糖;系统获得抗病性;TMV-CP基因;实时荧光定量PCR
摘  要：
采用实时荧光定量PCR方法,研究了壳寡糖诱导对烟草体内TMV-CP基因表达量的影响。结果表明,壳寡糖诱导后烟草表达了对TMV侵染的系统获得抗病性,植株显症推迟4~7 d,症状减轻,平均严重度比对照降低了82.9%。同时烟草叶片中CP基因的表达量显著降低。壳寡糖诱导处理的植株在接种病毒时(0 h),CP基因的表达量(拷贝数/2μL)为0.918,接种后168 h增加到167.730。而在不诱导对照植株内,CP基因的表达量在0 h为1.218,接种后168 h猛增到648.623。换言之,不处理对照的表达量在此期间增长了532.53倍,而壳寡糖诱导处理植株中仅增长了182.71倍。试验证明壳寡糖诱导的系统获得抗病性强烈抑制了烟草体内TMV-CP基因的表达。
译  名：
Inhibitory effect to TMV-CP gene expression in tobacco induced by chito-oligosaccharides
作  者：
SHANG Wen-jing1,WU Yun-feng1,SHANG Hong-sheng1,ZHAO Xiao-ming2,DU Yu-guang2(1College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture,Northwest A&F University,Yangling 712100,China;2 Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China)
关键词：
chito-oligosaccharides;systemic acquired resistance(SAR);TMV-CP gene;real-time quantitative RT-PCR
摘  要：
The inhibitory effect to TMV-CP gene expression in tobacco(Nicotiana tabacum) seedlings induced by chito-oligosaccharides was examined with real-time quantitative RT-PCR(QPCR).The results showed that high level systemic acquired resistance(SAR) was expressed in tobacco plants treated with solution of chito-oligosaccharides at the concentration of 50 μg/mL,the latent period was 4-7 d delayed and the average disease severity was 82.9% lowered.Meanwhile,the expression quantity(copies/2 μL) of CP gene in treated plants was reduced remarkably,it was 0.918 at 0 h(the time of virus inoculation) and 167.730 at 168 h after inoculation.But,in untreated plants the expression quantity was 1.218 at 0 h,and 648.623 at 168 h.During this time span that increased 532.53 times in untreated plants,and only 182.71 times in treated plants.It was concluded that the expression of TMV-CP gene in tobacco was strongly suppressed by SAR induced by chito-oligosaccharides.

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