作 者：
杨玉;孙煜;孙宝盛;卢营杰;李建慧;苗志强;张俊珍
关键词：
苜草素;产蛋后期蛋鸡;抗氧化;脂质代谢;基因表达
摘 要：
本试验旨在研究不同水平苜草素对产蛋后期蛋鸡抗氧化能力、脂质代谢及相关基因表达的影响。选用69周龄、健康海兰褐蛋鸡324只,随机分为3组,每组6个重复,每个重复18只鸡。对照组饲喂基础饲粮,试验组在基础饲粮的基础上添加500和1 000 mg/kg苜草素。试验预试期2周,正试期8周。结果表明,与对照组相比:1)试验组蛋鸡产蛋率显著升高(P<0.05),料蛋比显著降低(P<0.05)。2)500 mg/kg苜草素显著增加了蛋壳强度(P<0.05),极显著降低了肝脏甘油三酯含量(P<0.01),显著降低了肝脏总胆固醇含量(P<0.05)。3)1 000mg/kg苜草素极显著提高了血浆谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)及肝脏GSH-Px活性(P<0.01),显著提高了血浆总抗氧化能力(T-AOC)、降低了丙二醛(MDA)的含量(P<0.05),极显著降低了肝脏与蛋黄总胆固醇和甘油三酯含量(P<0.01),极显著提高了肝脏硫氧还蛋白还原酶1(Trx R1)、胆固醇-7α羟化酶(CYP7A1)、固醇调节元件结合蛋白-1c(SREBP-1c)mRNA表达量(P<0.01),极显著降低了3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)mRNA表达量(P<0.01),而对GSH-Px mRNA表达量无显著影响(P>0.05)。由此可见,饲粮中添加苜草素可降低产蛋后期蛋鸡蛋黄胆固醇和甘油三酯含量,提高生产性能、蛋壳强度、机体抗氧化和胆固醇代谢能力,且以添加1 000 mg/kg苜草素效果较好。
译 名：
Effects of Polysavone on Antioxidant Ability,Lipid Metabolism and Related Gene Expression of Laying Hens during Later Period of Laying
作 者：
YANG Yu;SUN Yu;SUN Baosheng;LU Yingjie;LI Jianhui;MIAO Zhiqiang;ZHANG Junzhen;College of Animal Science and Technology,Shanxi Agricultural University;
关键词：
laying hens;;polysavone;;antioxidant;;lipid metabolism;;gene expression
摘 要：
This experiment was to investigate the effects of different polysavone supplemental levels on the antioxidant ability,lipid metabolism and related gene expression of laying hens during later period of laying.Totally,three hundred and twenty-four Hy-Line Brown hens at 69-week old were randomly divided into 3 groups with 6 replicates and there were 18 hens for each replication.The hens in control group were fed a basal diet,and the two others were fed the basal diet supplemented with polysavone at the levels of 500 and 1 000 mg/kg,respectively.The preliminary experiment lasted for 2 weeks and the formal experiment lasted for 8 weeks.The results showed that compared with the control group,1) diet supplemented with polysavone significantly increased the laying rate and reduced the feed to egg ratio( P < 0.05).2) Diet supplemented with 500 mg/kg polysavone significantly increased the eggshell strength( P < 0.05),significantly decreased the liver triglyceride content( P < 0.01) and the cholesterol content( P < 0.05).3) Diet supplemented with 1 000 mg/kg polysavone extremely significantly increased the activities of plasma glutathion peroxidase( GSH-Px),total superoxide dismutase( T-SOD) and liver GSH-Px activity,as well as the expression quantity of thioredoxin reductase( Trx R1),cholesterol alpha-hydroxylase( CYP7 A1) and sterol regulatory element binding protein-1 c(SREBP-1c)(P < 0.01) and the plasma total antioxidant capacity(T-AOC)(P < 0.05),also significantly reduced the malondialdehyde( MDA) content in plasma( P < 0.05),contents of total cholesterol and triglyceride in liver and egg yolk and the expression quantity of 3-hydroxy-3-methyl glutaric acid acyl coenzyme A reductase( HMG CR)( P < 0.01).However,the addition of polysavone at the level of 1 000 mg/kg had no effects on the expression quantity of G SH-Px( P > 0.05).It can be concluded that diet supplemented with polysavone can reduce the cholesterol and triglyceride content in egg yolk,increase the growth performance,eggshell strength,antioxidant and lipid metabolism capacity of Hy-Line Brown hens during late period of laying and the 1 000 mg/kg polysavone is suggested as the optimal dosage in this study.
