Position: Home > Articles > Determination of Ribavirin,Ciprofloxacini Hidrochloridum,Trimethoprim Lactate in Compound Ribavirin Inhection by HPLC
Journal of Shanxi Agricultural Sciences
2003,31
(2)
87-89
兽用复方利巴韦林注射液组分含量色谱测定法
作 者：
赵拉柱;武晋孝;宋艳红;王仲琴
单 位：
山西省医药研究所;山西省兽药监察所
关键词：
复方利巴韦林注射液;利巴韦林;盐酸环丙沙星;乳酸甲氧苄胺嘧啶;高效液相色谱法;含量测定
摘 要：
采用高效液相色谱法测定兽用复方利巴韦林注射液各组分含量,以Extend-C18(十八烷基硅烷健合硅胶)为填充剂,水—乙腈—二乙胺(415∶85∶2.2,用磷酸调pH值为3.0)为流动相,流速1.0ml/min,检测波长225nm,进样量10μ1。该制剂利巴韦林在0.505～2.020μg、盐酸环丙沙星在0.510～2.040μg、乳酸甲氧苄胺嘧啶在0.250～1.000μg范围内峰面积与进样量呈线性关系,相关系数均为0.9999,平均回收率分别为99.9%,99.6%,99.5%。该方法分离效果好,准确可靠,适用于该产品质量控制。
译 名：
Determination of Ribavirin,Ciprofloxacini Hidrochloridum,Trimethoprim Lactate in Compound Ribavirin Inhection by HPLC
作 者：
ZHAO Lazhu1,WU Jinxiao2, SONG Yanhong2, WANG Zhongqin2(1.Shanxi Institute for MedcoPharmaceuty,Taiyuan 030001;2.Shanxi Institute for Vetcrinary Drug Control,Taiyuan 030027)
关键词：
HPLC;Compound ribavirin inhection;Ribavirin;Ciprofloxacni hidrochloridum;Trimethoprim lactate
摘 要：
To establish a method for determination of three constituents in compound ribavirin inhection:ribavirin,ciprofloxacini hidrochloridum and trimethoprim lactate by HPLC:Chromatograghic conditions were ZORBAX ExtendC18 column(4.6mm×150mm,5μm);mobile phase wateracetonitrilctriethylamine(415∶85∶2.2,adjust pH to 3.0 with H3PO4),;flow rate of 1.0ml/min.The UV detection wavelength was at 225 nm.Result:The linear response ranges were 0.505～2.020μg of ribavirin,0.510～2.040μg of ciprofloxacini hidrochloridum,0.250～1.000μg of trimethoprim lactate.The mean recoveries of ribavirin,ciprofloxacini hidrochloridum and trimethoprim lactate were:99.9%,99.6%,99.5%. Conclusion:The method shows good resolusion,satisfaetory accuracy.It is suitable for quality control of compound ribaverin inhection.
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