中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 一株鹅源巴氏杆菌强毒株的分离及鉴定 山东农业大学学报(自然科学版) 2012,43 (4) 522-528
Position: Home > Articles > ISOLATION AND IDENTIFICATION OF A VIRULENT STRAIN FROM GOOSE PASTEURELLA MULTOCIDA Journal of Shandong Agricultural University(Natural Science Edition) 2012,43 (4) 522-528

一株鹅源巴氏杆菌强毒株的分离及鉴定

作  者：
宋晓娜;刁有祥;高绪慧;葛平萍;张颖;鞠小军;王丽荣
单  位：
山东农业大学动物科技学院
关键词：
鹅;多杀性巴氏杆菌;分离鉴定;致病性
摘  要：
为了确定导致山东某鹅场种鹅发病的病原。从病死鹅肝脏、心脏中分离并纯化细菌,根据其形态特征、培养特性、生化鉴定、PCR和致病性试验等进行鉴定。致病性试验结果表明,1.2×109CFU/mL的菌液10倍系列稀释后,接种11日龄健康雏鸭后,该分离菌株对11日龄健康雏鸭的LD50为10-4.5CFU/mL。死亡雏鸭的剖检病变与病死鹅类似,并分离出形态特征完全一致的菌落。病理组织学变化以十二指肠绒毛断裂、脱落、肝细胞脂肪变性、心肌纤维断裂为特征。药敏试验结果显示,该分离菌株对头孢曲松钠、阿莫西林、氨苄西林、氧氟沙星等较敏感。山东某鹅场中发病病原经分离鉴定为多杀性巴氏杆菌,为禽巴氏杆菌病的诊断与防治提供了科学的依据。
译  名：
ISOLATION AND IDENTIFICATION OF A VIRULENT STRAIN FROM GOOSE PASTEURELLA MULTOCIDA
作  者：
SONG Xiao-na,DIAO You-xiang,GAO Xu-hui,Ge Ping-ping ZHANG Ying,JU Xiao-jun,WANG Li-rong(College of Animal Science and Technology,Shandon Agricultural University,Taian 271018,China)
关键词：
Goose;Pasteurella multocida;isolation and identification;pathogenicity
摘  要：
This study was conducted to confirm the pathogen of goose disease in as area of Shandong Province.A strain of goose Pasteurella multocida isola ted and purified from the livers,hearts of the sick breeding geese in an area of Shandong Province based on its morphological and culturd characteristics,biochemical test,PCR and Pathogenicity test and so on.Pathogenicity test showed that the median lethal dose was 10-4.5 CFU/mL of eleven day-old healthy ducklings after being were injected with the ten times diluted bacteria(1.2×109 CFU/mL).The pathological changes of the dead ducklings were similar to that of sick geese,and the strain which had the same morphological characteristics with Pasteurella multocida was reisolated.Histopathological changes were characterized by breaking and of intestinal villus in the duodenum,fatty degeneration in the liver cell and breakage of cardiac muscle fibers.Susceptibility test displayed that the strain was sensitive to ceftriaxone,amoxicillin,ampicillin,ofloxacin,and so on.The pathogen of goose disease was Pasteurella multocida anatis isolate in an area of Shandong Province.This provided scientific theory for diagnosing and preventing avian Pasteurella multocida.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46