关键词：
黑沙蒿;不同溶剂提取物;活性成分;自由基清除力
摘 要：
为了研究不同提取溶剂对黑沙蒿提取物得率、主要活性成分含量及其对自由基DPPH清除的影响,试验设5个处理,每个处理3个重复,各处理按1∶15(g/mL)料液比分别加入蒸馏水、乙醇、乙酸乙酯、乙醚和丙酮,通过超声波辅助法提取黑沙蒿中的生物活性成分;以维生素C为阳性对照,采用DPPH法来测定黑沙蒿不同溶剂提取物的抗氧化活性.结果显示:①与其他提取剂的提取物相比,蒸馏水提取物的得率最高(P<0.05),且其多糖和黄酮含量也最高(P<0.01),而乙醚提取物中多酚的含量最高(P<0.01).②同种溶剂提取物不同浓度相比,水提取物浓度为1 mg/mL时,对DPPH清除率极显著高于浓度为0、0.2和0.4 mg/mL时的清除率(P<0.01);乙醇提取物浓度为0.4和0.6 mg/mL、乙酸乙酯提取物浓度为1 mg/mL、乙醚提取物浓度为0.8 mg/mL、丙酮提取物浓度为0.6 mg/mL时,对DPPH的清除率均极显著高于浓度为0和0.2 mg/mL时的清除率(P<0.01).③与其他溶剂提取物相比,当提取物浓度为0.2 mg/mL时,无水乙醇提取物对DPPH的清除率极显著高于其他4种提取物对DPPH的清除率(P<0.01);提取物浓度为0.4和0.6 mg/mL时,水提取物对DPPH的清除率显著低于其他4种提取物对DPPH的清除率(P<0.01);浓度为0.8和1 mg/mL时,乙酸乙酯提取物对DPPH的清除率极显著高于其他4种提取物对DPPH的清除率(P<0.01).综上,黑沙蒿水提取物得率及多糖和黄酮含量最高,乙醚提取物中多酚含量最高,而无水乙醇和乙酸乙酯提取物抗氧化效果最好.
作 者：
WANG Junli;XING Yuanyuan;JIN Xiao;XU Yuanqing;SHI Binlin;College of Animal Science, Inner Mongolia Agricultural University;
关键词：
Artemisa ordosica;;different solvent extracts;;active component;;free radical scavenging power
摘 要：
The current study was conducted to study the effect of different extraction solvents on the yield of Artemisa ordosica extract, its main active component contents and its DPPH scavenging rate.The experiment consisted of 5 treatments with three replicates.Distilled water, ethanol, ethyl acetate, ether and acetone were added to each treatment at the material to solution ratio of 1:15(g/mL), and the bioactive components of A.ordosica were extracted by the ultrasonic assisted method.With vitamin C as the positive control, the DPPH method was used to determine the antioxidant activities of the extracts of artemisa ordosica by different solvents.The results showed as follows: ① When distilled water was used as the extraction agent, the extraction rate was the highest compared with the other extraction agents(P<0.05), and the content of polysaccharides and flavonoids were also the highest(P<0.01), but the content of polyphenol in the extract was the highest when ether was used as extraction agent(P<0.01).② Compared among different concentrations of the extracts by the same solvent, when distilled water was used as the extraction agent, the DPPH clearance rate at the concentration of 1 mg/mL was significantly higher than that at the concentration of 0, 0.2 or 0.4 mg/mL(P<0.01).When ethanol was used, the DPPH clearance rate at the concentrations of 0.4 and 0.6 mg/mL was significantly higher than that at the concentrations of 0 and 0.2 mg/mL(P<0.01).With ethyl acetate as the extraction agent, the DPPH clearance rate was significantly higher at the concentration of 1 mg/mL than at the concentrations of 0 and 0.2 mg/mL(P<0.01).When ethyl ether was used as the extraction agent, the DPPH clearance rate was significantly higher at 0.8 mg/mL than at 0 and 0.2 mg/mL(P<0.01).When acetone was used, the DPPH clearance rate at the concentration of 0.6 mg/mL was significantly higher than that at the concentrations of 0 and 0.2 mg/mL(P<0.01).③ Compared with the other solvent extracts, when the concentration of the extracts was 0.2 mg/mL, the DPPH scavenging rate of the anhydrous ethanol extract was significantly higher than that of the other four extracts ( P<0.01).At the concentrations of 0.4 and 0.6 mg/m L,the DPPH scavenging rate of the water extracts was significantly lower than that of the other four extracts( P<0.01).At concentrations of 0.8 and 1 mg/m L,the DPPH scavenging rate of ethyl acetate extract was significantly higher than that of the other four extracts( P<0.01).In short,the extraction rate of the water extract,and its contents of polysaccharides and flavonoids were the highest,but the contents of polyphenols were the highest in the ether extract,and the antioxidant effects of the ethanol and ethyl acetate extracts were the best.
