中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 重庆辣椒病毒病病原初步鉴定和分析 园艺学报 2015,42 (2) 263-270
Position: Home > Articles > Preliminary Identification and Analyses of Viruses Causing Pepper Virus Disease in Chongqing,China Acta Horticulturae Sinica 2015,42 (2) 263-270

重庆辣椒病毒病病原初步鉴定和分析

作  者：
郭思瑶;童艳;黄娅;罗信福;青玲
单  位：
西南大学含弘学院;西南大学植物保护学院
关键词：
辣椒;辣椒病毒病;Dot-ELISA;RT-PCR;病原鉴定
摘  要：
利用斑点免疫杂交法(Dot-ELISA)和RT-PCR法对重庆地区发生的辣椒病毒病的病原进行了鉴定和优势病毒分析。利用5种常见蔬菜病毒的血清对采自重庆8个区县的152份辣椒病毒病样品进行Dot-ELISA检测,结果表明,共有118份辣椒样品检测呈阳性,其中,黄瓜花叶病毒(Cucumber mosaic virus,CMV)的侵染最普遍,总检出率高达57.89%;番茄花叶病毒(Tomato mosaic virus,To MV)的总检出率最低,为22.52%;烟草花叶病毒(Tobacco mosaic virus,TMV)、芜菁花叶病毒(Turnip mosaic virus,Tu MV)和蚕豆萎蔫病毒2号(Broad bean wilt virus 2,BBWV-2)的总检出率分别为30.26%、36.18%和24.34%。在118份阳性样品中,有66.10%的样品受两种及以上病毒复合侵染,其中33.33%的样品受到CMV和Tu MV复合侵染,有26.27%的样品受3种病毒复合侵染。设计5种病毒的特异性引物,随机选取16份阳性样品进行RT-PCR扩增,克隆测序结果表明扩增片段确实是5种病毒的相应序列,且RT-PCR与ELISA检测结果基本吻合。检测结果表明CMV是重庆地区辣椒上的优势病毒种类,且该地区辣椒上病毒复合侵染现象普遍。本文首次报道了To MV和Tu MV侵染辣椒。
译  名：
Preliminary Identification and Analyses of Viruses Causing Pepper Virus Disease in Chongqing,China
作  者：
GUO Si-yao;TONG Yan;HUANG Ya;LUO Xin-fu;QING Ling;Hanhong College,Southwest University;College of Plant Protection,Southwest University;
关键词：
pepper;;pepper virus disease;;Dot-ELISA;;RT-PCR;;identification of pathogen
摘  要：
The viruses causing pepper virus disease in Chongqing,China were identified and analyzed by Dot-ELISA and RT-PCR. In this research,152 pepper samples with viral symptoms,collected from eight pepper-growing counties in Chongqing,were detected with the special serums against five common vegetable viruses by Dot-ELISA method. The detection results showed that 118 pepper samples were positive,among which the infection of Cucumber mosaic virus(CMV)is common,and the detection rate is 57.89%. The detection rate of Tomato mosaic virus(To MV)is the lowest,22.52%. The detection rate of Tobacco mosaic virus(TMV),Turnip mosaic virus(Tu MV),and Broad bean wilt virus 2(BBWV-2)were 30.26%,36.18% and 24.34%,respectively. Among the 118 positive samples,66.10% samples were mix-infected with more than two viruses,33.33% samples were infected with CMV and Tu MV,26.27% samples were infected with three viruses. Sixteen samples were randomly selected for RT-PCR amplification using specific primer pairs for the five viruses respectively,and the positive samples were then cloned and sequenced. The results showed that the amplified fragments were highly homologous with corresponding sequences of five viruses and the detection results were nearly consistent with those of ELISA detection. These results showed that CMV is the prevalent pathogenic virus,and the mix-infection of viruses on pepper was severe in Chongqing. This is the first report of To MV and Tu MV infecting pepper.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46